AccuPrep® His-tagged Protein Purification Kit

OverviewTechnical/SpecsLiterature/SupportOrdering Information

Fast micro scale purification of 6xHis-tagged protein

Bioneer's Ni-NTA magnetic silica resins are silica beads, with an average diameter of 1.29 um and a range of 1~5 um diameter, have Ni-NTA (nickel-nitrilotriacetic acid) covalently bound to their surface.
These Ni-NTA magnetic silica beads exhibit outstanding affinity to His-tagged proteins and feature our SSMB (Spherical-Shaped Magnetic Bead) technology that provides increased surface area (due to the small size of the beads), without the issue of impurity carryover common with magnetic particles that have a rough-surface.
Ni-NTA magnetic agarose beads are supplied as a 10% (v/v) suspension with an average binding capacity of 500 ug protein per ml of suspension for 6xHis-tagged protein.

Features and Benefits

Flexible: The protocol may be carried out with a magnet or via centrifugation.

Excellent performance: Highly pure proteins are obtained through our exclusive SSMB (Spherical-Shape Magnetic Beads).

High purity and High yield: Average binding capacity of 500 μg protein per ml of suspension for 6xHis-tagged protein. Purity that is > 90%.

Reproducibility: The only hands-on step is the addition of protein extract, maximizing reproducibility.

Fast process: The entire purification is complete in about 30 minutes!

Application

Figure 1. Comparison of Protein Tyrosine Phosphatase(25 kD) purified using AccuPrep® His-tagged Protein Purification Kit and two market leading competitors' products.
AccuPrep® His-tagged Protein Purification Kit outperformed two competitors' similar products in terms of yield and purity.
Lane M: AccuLadder™ Protein Size Marker (Broad)
Lane 1: Crude extract
Lane 2, 5: Bioneer
Lane 3, 6: Company Q
Lane 4, 7: Company E
Washing: Samples taken after the washing step
Elution: eluted protein samples

Figure 2. Various sized proteins purified using AccuPrep® His-tagged Protein Purification Kit.
Proteins as small as 10 kD and as large as 120 kD can be purified with the AccuPrep® His-tagged Protein Purification Kit.
Lane M: AccuLadder™ Protein Size Marker (Broad)
Lane 1: TLA DNA Polymerase
Lane 2: PTPase #54
Lane 3: PTPase pk6

Figure 3. Concentration comparison of TLA DNA polymerase proteins purified using AccuPrep® His-tagged Protein Purification Kit and two market leading competitors' products.
AccuPrep® His-tagged Protein Purification Kit showed up to 5-times higher concentration than that of the competitors.

Procedure

Manual

• AccuPrep® His-tagged Protein Purification Kit

MSDS

• AccuPrep® Ni-NTA binding-washing buffer

• AccuPrep® Ni-NTA Elution buffer

Brochure

• AccuPrep® His-tagged Protein Purification Kit

Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001:2008 - certificate

• EN ISO 13485:2003 / AC:2009 - certificate