For PCR, Reverse Transcription, RT-PCR and Ligation
Novel PCR polymerases with unique performance features
Bioneer offers a wide range of enzymes for PCR, Reverse Transcription, Reverse Transcriptase PCR (RT-PCR) and DNA Ligation. Our Top DNA Polymerase is the fastest thermostable PCR polymerase on the market. We are the only company to use a novel “enzyme-mediated” HotStart in our enzymes. In addition, our CycleScript Reverse Transcriptase uses a Cyclic-RT reaction that allows for robust cDNA synthesis of the rarest transcripts.
For faster and easier reaction set up, the AccuPower® PreMix Series contains each enzyme and all other PCR contents in an easy to resuspend lyophilized mix.
Features and Benefits
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Fast: Highly processive enzymes
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Flexible: for all applications from standard, to high fidelity and long range and more!
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Value: No license fees associated with our novel enzymes saves you money!
|
Application |
DNA Polymerase |
Hotstart DNA Polymerase |
ProFi Taq
DNA
Polymerase |
Reverse transcriptase |
Top |
Taq |
pfu |
Top |
Taq |
MMLV RT |
CycleScript RTase |
RocketScript Rtase |
PCR |
Standard PCR |
√ |
√ |
|
√ |
√ |
√ |
|
√ |
|
Hotstart PCR |
|
|
√ |
√ |
√ |
|
|
|
|
High-fidelity PCR |
|
|
√ |
|
|
√ |
|
|
|
Long range PCR |
|
|
√ |
|
|
√ |
|
|
|
High sensivity |
|
|
|
√ |
|
|
|
|
|
GC rich PCR |
|
|
|
√ |
√ |
|
|
|
|
Multiplex PCR |
|
|
|
√ |
√ |
|
|
|
|
RT |
Standard RT |
|
|
|
|
|
|
√ |
√ |
√ |
Cyclic RT |
|
|
|
|
|
|
|
√ |
|
Standard RT/PCR |
|
|
|
|
|
|
|
|
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Specifications of Bioneer Enzymes
Product Name |
Product size |
Yield |
Specificity |
Fidelity |
GC-rich |
Heat Stability |
Leaves 3'-A |
Top DNA Polymerase |
~ 10 kb |
***** |
**** |
*** |
*** |
*** |
Yes |
Taq DNA Polymerase |
~ 10 kb |
***** |
*** |
*** |
*** |
*** |
Yes |
Hotstart DNA Polymerase |
~ 12 kb |
**** |
**** |
*** |
**** |
**** |
Yes |
Hotstart Taq DNA Polymerase |
~ 12 kb |
**** |
**** |
*** |
**** |
**** |
Yes |
ProFi Taq DNA Polymerase |
~ 20 kb |
**** |
**** |
*** |
**** |
**** |
Yes |
Pfu DNA Polymerase |
~ 10 kb |
** |
*** |
**** |
** |
*** |
No |
M-MLV Reverse Transcriptase |
~ 9 kb |
*** |
- |
- |
*** |
** |
- |
CycleScript Reverse
Transcriptase |
~ 9 kb |
**** |
- |
- |
*** |
*** |
- |
RocketScript Reverse
Transcriptase |
~ 10 kb |
**** |
- |
- |
*** |
**** |
- |