• AccuTarget™ Predesigned siRNA Sets

siRNA libraries organized by gene family and function. 25 standard siRNA libraries and 21 fully validated siRNA libraries are available

Bioneer offers the AccuTarget™ Human siRNA Library which contains 25,368 predesigned and manufactured siRNAs for immediate use in your experiments. These Premade human siRNA library is available at 10, 20, 50 and 100 nmole guaranteed yield.. We also offer 25 Pathway specific / gene family siRNA library sets for researchers studying cellular processes, cancer, disease etc. These are available at 0.1, 0.25, 0.5 and 1 nmole guaranteed yield. Finally there are 21 pre-validated siRNA libraries with high knockdown rates and demonstrated effectiveness. Validated siRNAs can be ordered at 10, 20, 50 and 100 nmole guaranteed yield.

Features and Benefits

 

• Categorized by Pathway / family: Convenient format for research

• Pre-validated siRNA libraries available: Works the first time and every time

• Competitive pricing: Great value for your research dollar

Bioneer provides various libraries categorized by gene family and/or pathway. All siRNAs are synthesized in our state-of-the-art clean room facility and then purified free of charge utilizing the company's Bio-RP purification system. Each siRNA is quality controlled by MALDI-TOF mass spectrometry to guarantee highest quality and analyzed by PAGE to confirm its duplex structure. (Figure 1 and 2)

Figure 1. MALDI-TOF mass spectrometry analysis of a custom siRNA. All siRNAs are processed by MALDI-TOF mass spectrometry to ensure its quality.


Figure 2. PAGE data of double-stranded custom siRNA. Complementary single-stranded RNA strands were hybridized to form siRNA duplex and analyzed by 15% non-denaturing PAGE.
SS: single-stranded RNA
DS: double-stranded siRNA               



Figure 3. Knockdown efficiency of AccuTarget™ siRNA Library. To determine the knockdown efficiency, HeLa cells were transfected with individual siRNAs at 100 nM concentration. 24 hours post-transfection, total RNA was isolated and the level of target mRNA was measured by qRT-PCR. This data demonstrates the effectiveness of the Turbo si-Designer algorithm: 83.8% of tested siRNAs induced > 70% knockdown and 38.1% of tested siRNAs elicited > 90% knockdown.



Figure 4. Knockdown efficiency of AccuTarget™ Human Validated Cell Cycle siRNA set. HeLa cells were transfected with individual siRNAs targeting 33 different genes at a concentration of 20 nM. Total RNAs was isolated and the level of target mRNA was measured by qRT-PCR. This data demonstrates the effectiveness of the Turbo si-Designer algorithm.

Manual

• siRNA manual

Brochure

• siRNA Products and services

Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001:2008 - certificate

• EN ISO 13485:2003 / AC:2009 - certificate

• EC Directive 98/79/EC - certificate