ExiProgen™ Rice Genomic DNA Kit

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Automatic Genomic DNA Extraction Kit – ExiProgen™ Rice Genomic DNA Extraction Kit

ExiProgen™ Rice Genomic DNA Kit
ExiProgen™ Rice Genomic DNA Kit uses our unique lysis buffer with Proteinase K to efficiently lyse the rice seed for efficient extraction of genomic DNA. The binding buffer and silica magnetic particles will bind genomic DNA to the surface of the beads. The wells are then subject to a magnetic field, forcing the genomic DNA-bound silica magnetic particles to remain within the container while the reaction mixture and cellular waste are removed. Three types of washing buffers are introduced sequentially during in the next step to remove the remainder of cellular waste products. The washing buffers are then pipetted off, while the genomic DNA-bound silica magnetic particles remain bound by the magnetic field. DNase-free elution buffer is then introduced to the silica magnetic particles to release the bound and now purified genomic DNA.

Features and Benefits

Reproducibility & Reliability

ExiProgen™ Rice Genomic DNA Kit functions through a buffer cartridge system containing buffers optimized for the extraction of genomic DNA from rice sample. Nucleic acid extractions occur with the buffer cartridges through the automated nucleic acid extraction instrument, allowing for reproducible performance. Also, by preventing human errors during the workflow, the results are always dependable. The product is produced under ISO 9001 quality standards by an automated production facility to provide you with high-quality performance.

High-Yield and Purity

The buffer composition within the ExiProgen™ Rice Genomic DNA Kit was developed to extract high purity and high yield genomic DNA from rice sample. Also, the extraction protocols contained within the automated nucleic acid extraction instruments ExiProgen™ is optimized for maximum performance. Anybody can extract genomic DNA of high purity and yield from rice sample by using the pre-programmed and optimized protocols in ExiProgen™.

Contents

Components	Quantity	Note
Buffer Cartridge ①	6 ea
Buffer Cartridge ②	6 ea
Rice Lysis buffer	1 ea	40 ml
Rice Binding buffer	1 ea	20 ml
Proteinase K, lyophilized	2 tube	20 mg/ tube
Disposable filter tip	3 pack	32 ea/pack
Elution tube	1 pack	12 ea/pack
Manual	1 ea

Specifications

Starting culture volume	10 - 20 mg
Elution volume	50 ul
Expected Yield	1-2 ug
Expected Purity (A260/280)	> 1.8

Applications

PCR, Quantitative real time PCR, Gene cloning, SNP analysis, Genetics

Procedure

Experimental Data

Fig. 1 Agarose gel electrophoresis results of genomic DNA extracted from rice sample
Results of gel electrophoresis of genomic DNA extracted from rice sample. Yields were on average 1-2ug and purities (A₂₆₀/A₂₈₀) were at least (average) 1.8.

Fig. 2 Multiplex conventional PCR results of genomic DNA extracted from rice sample
Results of gel electrophoresis of PCR product using its specific PCR primer sets to distinguish its cultivar varieties.