AccuPower® Greenstar™ qPCR PreMix

The Real-Time PCR Reagents for the SYBR® Green-Based Detection



The Greenstar™ qPCR PreMix provides significant reduction of nonspecific reaction, high sensitivity, extended stability and great universality. Just additions of primers of your interesting target gene into Greenstar™ qPCR PreMix provide always reproducible results with convenience of use.

Features and Benefits

• High Specificity: A Non-specific reaction by Enzyme-mediated Hotstart Technology was dramatically eliminated(patent 292883 & 10-2007-109055)
• User Convenience:  Just add primer for target gene.
• Extended Stability: In a freezer, the activity of this product is stable for 2 years.
• Reproducibility: Margin of error was minimized and reproducible results can be obtained, because no extra mixing step is requied.
• Equipment Compatibility: Optimized results on various Real-Time PCR instruments can be obtained.
• Universality of target gene: Significant results can be obtained regardless of kinds of target genes.

Specifications

5' to 3' exonuclease: No
3' to 5' exonuclease: No
3’ – A Overhang: Yes
Fragment Size: ~1 kb

Application

- Real-Time Quantification of DNA/cDNA targets.
- Quantification of gene expression
- Microbial/Viral Pathogen Detection.


Figure 1. Real-Time PCR Data of AccuPower® Greenstar™ qPCR PreMix

AccuPower® Greenstar™ PreMix provides at least 7 orders of magnitude in dynamic range (10 fg ~ 10 ng /reaction).
A : Amplification curve. Lambda DNA primers were added into Greenstar™ qPCR PreMix. A series of Lambda DNA positive control diluents were tested.
B : Standard curve. Data were obtained using Exicycler™ 96 Real-Time Quantitative Thermal Block (Bioneer Co.).
C : Melting curve.

Figure 2. Comparison of amplification quality between AccuPower® Greenstar™ qPCR PreMix and other supplier's master mixture.

Amplification & Standard curve of AccuPower® Greenstar™ qPCR PreMix and other supplier’s master mixture.
Lambda DNA primers were added into AccuPower® Greenstar™ qPCR PreMix and other supplier’s master mixture. A series of Lambda DNA positive control diluents were tested. Reaction mixtures were prepared and qPCR was performed according to each supplier’s protocol.
All data were obtained using ABI 7500 Fast Real-Time PCR machine (Applied Biosystems co.).

Notice to Purchaser

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

The lyophilized composition of this product is US Patent Nos. 5861251 and 6153412. Certain applications of this product are covered by pending or issued patents in certain countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used. Products may be covered by pending or issued patents. Please contact us for more information.

Note: 'Top DNA Polymerase' of this product is specifically optimized for increasing base incorporation rate by inactivating 5'->3' exonuclease activity. Therefore, this is not recommended to use for Real Time PCR using Taqman Probe, for which AccuPower® Greenstar™ qPCR PreMix.

Trademark: 'AccuPower®' is a registered trademark of Bioneer Corporation. 'DualStar™' is a trademark of Bioneer Corporation.

* Some of the products may not be available in some countries, For details, please contact your local distributor.

Manual
• AccuPower® Greenstar™ qPCR PreMix

MSDS
• AccuPower® Greenstar™ qPCR PreMix

Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001:2008 - certificate

• EN ISO 13485:2003 / AC:2009 - certificate