AccuPower® PreMix series is an innovative PCR master mix product range designed to make your PCR, reverse transcription (cDNA amplification) and RT-PCR experiments easier and more cost effective than ever. With a wide range of applications and unparalleled ease of use and stability, AccuPower® PreMix PCR master mix series will be an important addition to your collection of research tools.
Each tube or plate you order has all the components of a PCR master mix (enzyme, buffer, dNTPs) lyophilized with a patented stabilizer that maintains full activity for over one month at room temperature and up to two years in the freezer. Simply add your primers and template – and never make a dNTP or PCR enzyme stock solution again!
In addition, AccuPower®’s reaction stability is maintained at 95°C for up to 3 times longer than Taq DNA polymerase, making it an ideal enzyme for amplifying G:C rich DNA at temperatures above 50°C.
AccuPower® PreMix PCR master mix is available with or without loading dye. When loading dye is present you can load it directly into your gel following PCR – no glycerol or glucose is required.
Features and Benefits
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Fast: just add primer and template
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Convenient: no more dNTP or enzyme stock solutions required
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Flexible: available in tube (0.2 or 0.5 ml) or plate (96 or 384 well) formats
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Stable: for up to 2 years in the freezer and one month at room temperature
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Customizable: available with primers already added for even greater convenience
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Gel loading : available with or without tracking dye for ease of use
DNA Amplification
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Amplification |
PCR PreMix |
Taq PCR PreMix |
Hotstart PCR PreMix |
Pyro Hotstart Taq PCR PreMix |
Pfu PCR PreMix |
Multiplex PCR PreMix |
Gold Multiplex PCR PreMix |
PCR |
Standard PCR |
√ |
√ |
√ |
√ |
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√ |
√ |
Hot-start PCR |
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√ |
√ |
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√ |
√ |
High-fidelity PCR |
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√ |
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Long-range PCR |
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GC rich PCR |
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√ |
√ |
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Real-Time PCR |
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Multiplex PCR |
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√ |
√ |
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√ |
√ |
RNA Amplification
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Amplification |
RT PreMix |
Cycle Script RT PreMix |
Rocket Script RT PreMix |
Rocket Script Cycle RT PreMix |
RT/PCR PreMix |
RocketScript RT/PCR PreMix |
Rocket Plex RT/PCR PreMix |
PCR |
Standard PCR |
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√ |
√ |
√ |
RT |
Standard RT |
√ |
√ |
√ |
√ |
√ |
√ |
√ |
Cycle RT |
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√ |
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√ |
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√ |
√ |
RT/PCR |
Standard RT/PCR |
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√ |
√ |
√ |
Multiplex RT/PCR |
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√ |
Real time PCR
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Amplification |
GreenStar
qPCR PreMix |
2X GreenStar
qPCR master Mix |
DualStar
qPCR PreMix |
PCR |
Standard PCR |
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Hot-start PCR |
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High-Fidelity PCR |
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|
|
Long-range PCR |
|
|
|
GC rich PCR |
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|
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Real-Time PCR |
√ |
√ |
√ |
MultiplexPCR |
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Specifications of AccuPower PreMix series
Product |
Product Size |
Yield |
Specificity |
Fidelity |
Conven-
ience |
GCrich |
Heat
Stability |
Lambda |
Human |
PCR PreMix |
≤ 10 kb |
≤ 3 kb |
***** |
*** |
*** |
***** |
*** |
*** |
Taq PCR PreMix |
≤ 10 kb |
≤ 3 kb |
***** |
*** |
*** |
***** |
*** |
*** |
Hotstart PCR PreMix |
≤ 12 kb |
≤ 3 kb |
**** |
**** |
*** |
***** |
**** |
**** |
PyroHotstart Taq PCR PreMix |
≤ 12 kb |
≤ 3 kb |
**** |
**** |
*** |
***** |
**** |
**** |
Pfu PCR PreMix |
≤ 20 kb |
≤ 10 kb |
*** |
*** |
**** |
***** |
*** |
*** |
ProFi Taq PCR PreMix |
≤ 30 kb |
≤ 21 kb |
*** |
**** |
***** |
***** |
*** |
**** |
Multiplex PCR PreMix |
- |
1~20 frag. |
**** |
**** |
*** |
***** |
*** |
**** |
Gold Multiplex PCR PreMix |
- |
1~20 frag. |
**** |
**** |
*** |
***** |
*** |
**** |
RT PreMix |
- |
< 9 kb |
*** |
- |
- |
***** |
*** |
*** |
CycleScript RT PreMix |
- |
< 9 kb |
**** |
- |
- |
***** |
*** |
*** |
RocKetScript™ RT PreMix |
- |
< 10kb |
**** |
- |
- |
***** |
**** |
**** |
RocKetScript™ Cycle RT PreMix |
- |
< 10kb |
**** |
- |
- |
***** |
**** |
**** |
RT/PCR PreMix |
- |
< 6 kb |
**** |
- |
- |
***** |
*** |
*** |
RocKetScript™ RT/PCR PreMix |
- |
< 6 kb |
**** |
- |
- |
***** |
*** |
**** |
RocketPlex RT/PCR PreMix |
- |
< 6 kb |
**** |
**** |
- |
***** |
*** |
**** |
GreenStar™ qPCR PreMix |
- |
- |
*** |
**** |
- |
***** |
**** |
**** |
2X GreenStar™ qPCR master Mix |
- |
- |
*** |
**** |
- |
***** |
**** |
**** |
DualStar™ qPCR PreMix |
- |
- |
*** |
***** |
- |
***** |
**** |
**** |