AccuPower® Plus Dualstar™ qPCR PreMix

Best real time PCR kits for Probe – AccuPower® Plus Dualstar™ qPCR PreMix, 2X Master Mix

AccuPower® Plus Dualstar™ qPCR PreMix is a kit for providing accurate data from your various samples speedily. There are no additional mixing steps because it already prepared and dried for your convenient. This product makes you monitor your gene amplification of Ct in every steps of amplified florescent cycle because we add “probe” which include florescence oligo-nucleotide and primers. In addition, AccuPower® Plus Dualstar™ qPCR PreMix can store for two years in your -20°C freezer and you satisfy with this because we provide several different types of tube and plate depends of various qPCR machine.

Features and Benefits

• User Convenience: For this you can add probe and primers for your target gene simply. In addition, you can satisfy your result by using this kit for several different types of qPCR machine.
• Dynamic Range: There are wide ranges of copies like 8 log from 10 to 108 copies.
• Specificity: You can get the best amplification result with your target gene by using Hotstart Taq DNA polymerase 
• Universality of target gene: You always achieve great real time PCR result even though you use various types of DNA, cDNA, high GC template and more.
• Easy-to-use: In this kit, we provide the product formation like freeze-dried premix or master mix type included thermostable DNA polymerase, dNTPs and provide separately and individually. Therefore, the users add only Template, Primers and DEPC distilled water when you run your real time PCR.
• Reproducibility: Under ISO 9001 Quality Assurance System, our mass produced product AccuPower® Plus Dualstar™ qPCR PreMix, 2X Master mix give you great satisfactory with uniform amplified effectiveness in all your reaction tube. 
   

5' to 3' exonuclease activity: Yes
3' to 5' exonuclease activity: No
3’ – A Overhang: Yes
Fragment Size: -

Application

- Gene expression profiling
- Target DNA quantification
- Microbial detection
- Viral/bacterial pathogen load determination
- Evaluation of primer pair performance for probe-based real-time PCR

Storage Temperature

-20°C

Experimental Data



Figure 1. High specificity of AccuPower® Plus DualStar™ qPCR PreMix



Figure 2. Comparison of amplification quality between AccuPower® Plus DualStar™ qPCR PreMix and other supplier's Real time qPCR kit.

Figure 3. Five-target multiplexing on the Exicycler™ instrument using AccuPower® Plus DualStar™ qPCR PreMix
Figure 3 shows amplification of a 5-target multiplex assay. The dyes used were FAM, TET, CY5, Texas Red and TAMRA, respectively. The data demonstrate that over a dilution series of input template, the AccuPower® Plus DualStar™ qPCR PreMix can successfully and reliably generate up to 5-target multiplex data on the Exicycler™.

Figure 4. PCR inhibitor study using AccuPower® Plus DualStar™ qPCR PreMix

Manual
• AccuPower® Plus Dualstar™ qPCR PreMix

MSDS
• AccuPower® Plus Dualstar™ qPCR PreMix

Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001:2008 - certificate

• EN ISO 13485:2003 / AC:2009 - certificate