ExiProgen™ Tissue Genomic DNA Kit

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For various animal tissue samples, Automatic Genomic DNA Extraction Kit – ExiProgen™ Tissue Genomic DNA Extraction Kit

ExiProgen™ Tissue Genomic DNA Kit
ExiProgen™ Tissue Genomic DNA Kit uses our unique Tissue lysis buffer with Proteinase K to efficiently dissociate tissues and proteins for efficient extraction of genomic DNA. The binding buffer and silica magnetic particles will bind genomic DNA to the surface of the beads. The wells are then subject to a magnetic field, forcing the genomic DNA-bound silica magnetic particles to remain within the container while the reaction mixture and cellular waste are removed. Three types of washing buffers are introduced sequentially during in the next step to remove the remainder of cellular waste products. The washing buffers are then pipetted off, while the genomic DNA-bound silica magnetic particles remain bound by the magnetic field. DNase-free elution buffer is then introduced to the silica magnetic particles to release the bound and now purified genomic DNA.

Features and Benefits

Reproducibility & Reliability

ExiProgen™ Tissue Genomic DNA Kit functions through a buffer cartridge system containing buffers optimized for the extraction of genomic DNA from various animal tissue samples. Nucleic acid extractions occur with the buffer cartridges through the automated nucleic acid extraction instrument, allowing for reproducible performance. Also, by preventing human errors during the workflow, the results are always dependable. The product is produced under ISO 9001 quality standards by an automated production facility to provide you with high-quality performance.

High-Yield and Purity

The buffer composition within the ExiProgen™ Tissue Genomic DNA Kit was developed to extract high purity and high yield genomic DNA from various animal tissue samples. Also, the extraction protocols contained within the automated nucleic acid extraction instruments ExiProgen™ is optimized for maximum performance. Anybody can extract genomic DNA of high purity and yield from animal tissue samples by using the pre-programmed and optimized protocols in ExiProgen™.

Contents

Components	Quantity	Note
Buffer Cartridge ①	6 ea
Buffer Cartridge ②	6 ea
Tissue lysis buffer	1 ea	25 ml
Disposable filter tip	3 pack	32 ea/pack
Elution tube	1 pack	12 ea/pack
Manual	1 ea

Specifications

Starting culture volume	up to 25 mg
Elution volume	50 - 100 ul
Expected Yield	5 - 20 ug
Expected Purity (A260/280)	> 1.8

Applications

PCR, Quantitative real time PCR, Gene cloning, SNP analysis, Genetics

Procedure

Experimental Data

Fig. 1 Agarose gel electrophoresis results of genomic DNA extracted from mouse tissue
Results of gel electrophoresis of genomic DNA extracted from 25mg mouse lung tissue. Yields were on average 15-20ug and purities (A₂₆₀/A₂₈₀) were at least (average) 1.8. Also, to observe cross-contamination that may occur during the extraction process, the mouse tissue samples were placed in a checkerboard pattern, and the rest of the wells were filled with D.W. No cross-contamination could be detected. M; Size marker, S; Extraction with mouse tissue sample, B; Extraction with D.W only.

Fig. 2 Agarose gel electrophoresis results of genomic DNA extracted from mouse tail tip
Results of gel electrophoresis of genomic DNA extracted from mouse tail tip. Yields were on average up to 15ug and purities (A₂₆₀/A₂₈₀) were at least (average) 1.8. Also, to observe cross-contamination that may occur during the extraction process, the mouse tail tip samples were placed in a checkerboard pattern, and the rest of the wells were filled with D.W. No cross-contamination could be detected. M; Size marker, S; Extraction with mouse tail tip sample, B; Extraction with D.W only.