AccuPower® Dualstar™ qPCR PreMix

The Real-Time PCR Reagents for the Sequence-specific Probe-Based Detection



The Dualstar™ qPCR PreMix provides significant reduction of nonspecific reaction, high sensitivity, extended stability and great universality. Just additions of primers and fluorescent dye labeled probe of your interesting target gene into Dualstar™ qPCR PreMix provide always reproducible results with convenience of use.

Features and Benefits

• User Convenience: Just add probe and primer for target gene.
• Extended Stability: In a freezer, the activity of this product is stable for 2 years.
• Reproducibility: Margin of error was minimized and reproducible results can be obtained, because no extra mixing step is requied.
• High Specificity: A Non-specific reaction by Enzyme-mediated Hotstart Technology was dramatically eliminated(patent 292883 & 10-2007-109055)
• Equipment Compatibility: Optimized results on various Real-Time PCR instruments can be obtained.
• Universality of target gene: Significant results can be obtained regardless of kinds of target genes.

Specifications

5' to 3' exonuclease: Yes
3' to 5' exonuclease: No
3’ – A Overhang: Yes
Fragment size: ~1 kb

 

Application

Real-Time quantification of DNA and cDNA targets, gene expression profiling, microbial & viral pathogen detection, evaluation of probe-based real-time PCR system


Figure 1. Real-Time PCR Data of AccuPower® Dualstar™ qPCR PreMix

AccuPower® Dualstar™ qPCR PreMix provides at least 7 orders of magnitude in dynamic range (10⁷ ~ 10¹ copies/reaction).
A : Amplification curve. West Nile Virus (WNV) primers and TaqMan-based probe were added into Dualstar qPCR Premix.
A series of WNV positive control diluents were tested.
B : Standard curve. All data were obtained using Exicycler™ 96 Real-Time Quantitative Thermal Block (Bioneer Co.).


Figure 2. Data using various kinds of Real-Time PCR Instruments (ABI 7500 Fast, ABI 7500, Opticon Real-Time PCR instruments)

AccuPower® Dualstar™ qPCR PreMix provides at least 7 orders of magnitude in dynamic range (10⁷ ~ 10¹ copies/reaction).
A : Amplification curve. West Nile Virus (WNV) primers and TaqMan-based probe were added into Dualstar qPCR Premix.
A series of WNV positive control diluents were tested.
B : Standard curve. All data were obtained using Exicycler™ 96 Real-Time Quantitative Thermal Block (Bioneer Co.).

Notice to Purchaser

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

The lyophilized composition of this product is covered by US Patent Nos. 5861251 and 6153412 of Bioneer Corporation. Certain applications of this product are covered by pending or issued patents in certain countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used. Products may be covered by pending or issued patents. Please contact us for more information

Trademark: 'AccuPower®' is a registered trademark of Bioneer Corporation. 'DualStar™' is a trademark of Bioneer Corporation.

* Some of the products may not be available in some countries, For details, please contact your local distributor.

Manual
• AccuPower® Dualstar™ qPCR PreMix

MSDS
• AccuPower® Dualstar™ qPCR PreMix

Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001:2008 - certificate

• EN ISO 13485:2003 / AC:2009 - certificate

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Cat.No.	Product Description	Price	Add to Cart
K-6100	AccuPower® DualStar™ qPCR PreMix, Exicycler 8-well strip, 20 µl, 12 strips, optical film included	Inquire
K-6101	AccuPower ® DualStar™ qPCR PreMix, ABI 7500 8-well strip, 20 µl, 12 strips, optical film included	Inquire
K-6102	AccuPower ® DualStar™ qPCR PreMix, 20 µl, 12 strips, Opticon 8-well strip	Inquire
K-6103	AccuPower® DualStar™ qPCR PreMix, Exicycler 96-well plate, 20 µl, 1 plate, optical film included	Inquire
K-6104	AccuPower ® DualStar™ qPCR PreMix, ABI 7500 96-well plate, 20 µl, 1 plate, optical film included	Inquire