ExiProgen™ EC-Maxi Protein Synthesis Kit

ExiProgen™ EC-Maxi Protein Synthesis Kit enables it to synthesize large scale of protein using the patented SECF (Stepwise Exchange Cell-Free) Protein Synthesis technology.
 

This kit is for automated large scale synthesis of a target protein using the patented SECF(Stepwise Exchange Cell-Free) protein synthesis technology. After adding template DNA into ExiProgen™ and choosing protocol No. 903, up to 500 µg of target protein can be automatically synthesized within a day. All processes including protein synthesis and dialysis will be performed in an automated fashion and the final protein harvested in a storage solution of choice.

Features and Benefits

Fully automatic: DNA-in-Protein (in storage buffer)-out
By simply installing 1-10 µg of template DNA into ExiProgen™, the target protein is synthesized and harvested in
any storage buffer of choice.

Parallel processing
1-8 different proteins can be synthesized in a single run.

High purity and high yield
Up to 500 ug of protein can be obtained per well with a purity of >90%.

Procedure and principle

This kit is designed for automated large scale expression of a target protein using patented SECF technology using ExiProgen™. It utilizes cell-free protein synthesis and purification using Ni-NTA magnetic beads. As it provides energy source in a stepwise manner into reaction tubes, the yields are maximized.

As described above, template DNA is mixed in a reaction tube with E. coli extract and Master mix. The reaction tube is installed into ExiProgen™' s reaction block along with other reagents. Finally, choose protocol and initiate run.

ExiProgen™ begins to synthesize the target protein by providing the energy source in stepwise manner. The synthesized target protein is then purified via Ni-NTA magnetic beads.

Experimental results

1. Up to eight proteins with various sizes can be synthesized in a single run.

M1; AccuLadder™ Protein Size Marker (Low)
1; CalmL3 (17.5kDa) , 2; DUSP 3 (22kDa),
3; CAT (24kDa), 4; AcGFP (29kDa),
5; RFP(30 kDa) 6; EF-Ts (34kDa),
7; VF (45kDa), 8; BM3 (117kDa)
M2; AccuLadder™ Protein Size Marker (Broad)
* Amount of loading sample is 1/80 of total sample.

Note) Bioneer highly recommends using plasmid DNA containing target gene for this kit.

Kit contents and storage conditions

This kit consists of two separate boxes, and its components are described in the following table.

Manual

Manual ExiProgen™ EC-Maxi Protein Synthesis Kit

MSDS

MSDS ExiPrep™ EC-Maxi Protein Synthesis Kit

Brochure

Brochure ExiProgen™ EC-Maxi Protein Synthesis Kit

Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001:2008 - certificate

• EN ISO 13485:2003 / AC:2009 - certificate

Cat.No.	Product Description	Price	Add to Cart
K-7310	ExiProgen™ EC-Maxi Protein Synthesis Kit, 8 reactions	Inquire