Lambda DNA / Hind III Marker

Lambda DNA/Hind III DNA Marker is generated by digesting lambda DNA with restriction enzyme Hind III. The resulting DNA Ladder is then purified, and buffer and dye are added so the marker is ready to load. Lambda DNA/Hind III Marker consist of 8 double stranded Lambda DNA fragments ranging in size from 125 to 21,226 bp, making it an ideal molecular weight marker for daily use.

Note

The DNA marker can be applied directly onto an agarose gel.
The cohesive ends (indicated by an * in the picture above) of the 12 nt cos site of bacteriophage lambda may anneal and form an additional band. These fragments can be separated by heating at 60 - 65°C for 5 minutes and then cooling on ice for 3 minutes.

Features and Benefits

• Complete and ready to load: Convenient

• Uniform sample concentrations: Can be used for sample quantitation

• Competitive pricing: Great value for your research dollar

Specifications

Concentration: 0.2 ug/μl
Recommended loading: 2.5 μl / 5 mm lane width
Typical Number of lanes: 200 (5 mm lane width)
Size range (bp): 125 - 23,130
Number of bands: 8
Supplied in: 10 mM Tris-HCl (pH 8.0), 1 mM EDTA, 2.5% Ficoll, 0.005% Bromphenol Blue, 0.005% Xylene Cyanol
Storage: -20°C



0.7% TAE agarose gel stained with Ethidium Bromide.

Manual

• Lambda DNA/Hind III Markers

MSDS

• Lambda DNA/Hind III Marker

Brochure

• DNA Ladders and Makers

Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001:2008 - certificate

• EN ISO 13485:2003 / AC:2009 - certificate