AccuPower® RT PreMix

Ready-to-use PCR master mix for cDNA synthesis from total RNA

The AccuPower® RT PreMix is a master mix for cDNA synthesis that consists of an easy to resuspend, lyophilized mix of M-MLV Reverse Transcriptase, RNase inhibitor, dNTPs, reaction buffer, tracking dye, and patented stabilizer. The master mix kit is used for first strand cDNA synthesis from RNA. Downstream applications include cDNA amplification with reverse transcription PCR. All of the key components are premixed at optimal concentrations. Simply add template RNA, primers and water to start your reaction.

For cDNA amplification with Cyclic RT, please see our AccuPower® CycleScript RT PreMix.
 

Features and Benefits

•  Convenient lyophilized RT: Easy to use, simply add your purified RNA
•  High yield of cDNA: For genes up to 9 kb within 10 minutes
•  Stable for 2 years at -20°C: Long life
•  RNase, DNase and Proteinase-free: Ensures the integrity of your samples

 

Specifications

5' to 3' exonuclease: No
3' to 5' exonuclease: No
3’ – A Overhang: No
Fragment size: 9 kb

 

Application

cDNA synthesis



Figure 1. Reliability and reproducibility test with AccuPower® RT PreMix. Each template showcases amplified target genes.

Lane M: 100 bp DNA Ladder (D-1030)
Lane 1-4: Reliability test of each lot with AccuPower® RT PreMix

 

Manual
• AccuPower® RT PreMix

MSDS
• AccuPower® PCR Master Mixes

Brochure
• AccuPower® PCR Master Mixes - 2010 Brochure

Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001:2008 - certificate

• EN ISO 13485:2003 / AC:2009 - certificate

Cat.No.	Product Description	Price	Add to Cart
K-2040	AccuPower® RT PreMix, 0.5ml thin-wall tubes with attached cap / 100 tubes, 20 μL reaction	Inquire
K-2041	AccuPower® RT PreMix(-dye), 0.2 ml thin-wall 8-strip tubes with attached cap / 96 tubes, 20 ul reaction	Inquire
K-2041-B	AccuPower® RT PreMix, 0.2 ml thin-wall 8-strip tubes with attached cap / 480 tubes, 20 ul reaction	Inquire
K-2042	AccuPower® RT PreMix, 0.5 ml thin-wall tubes with attached cap / 100 tubes, 50 ul reaction	Inquire
K-2043	AccuPower® RT PreMix, 0.2 ml thin-wall 8-strip tubes with attached cap / 96 tubes, 50 ul reaction	Inquire