For cDNA Synthesis, RT-PCR, and qRT-PCR
Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase is an RNA-dependent DNA polymerase. This enzyme is able to use an RNA molecule as a template and synthesize a double-stranded DNA.
M-MLV Reverse Transcriptase is isolated from an
E.coli strain containing a recombinant clone. It is ideal for use of first-strand synthesis cDNA from RNA molecules and for cDNA synthesis for Reverse Transcriptase PCR and qRT-PCR
Features and Benefits
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Optimized 5X buffer: For more full length fragments - within 10 minutes
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Full length cDNA: For genes up to 9 kb
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RNase, DNase and Proteinase-free: Ensures the integrity of your samples
Enzyme Properties
5' to 3' exonuclease activity: No
3' to 5' exonuclease activity: No
3’ – A Overhang: No
Strand Displacement: Yes Fragment Size: Up to 9 kb
Application
First strand cDNA synthesis from RNA, RT-PCR, and qRT-PCR
Reagents Supplied
5 x Reaction Buffer: 50 mM Tris-HCl, 250 mM KCl, 10 mM MgCl2, pH 8.1
100 mM DTT
dNTP Mix: 2.5 mM of each dNTP
Concentration
200 U/μl
Storage Conditions
50% glycerol containing 20 mM Tris-Cl (pH7.6), 150 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1 % IGEPAL CA-630
Store Temperature
-20°C
Unit Definition
One unit is defined as the amount of enzyme required to incorporates 1 nmole of dTTP into acid-precipitable material in 10 minutes at 37°C using poly (A)oligo (dT) as template primer.
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Cat.No.
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Product Description
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Price
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Add to Cart
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| E-3121
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M-MLV Reverse Transcriptase, 10,000 U, 5 X Reaction Buffer, 0.5 mL, 100 mM DTT, 0.3 mL, 10mM dNTPs mixture solution 0.2ml
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Inquire
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| E-3122
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M-MLV Reverse Transcriptase, 50,000 U, 5 X Reaction Buffer, 2.5 mL, 100 mM DTT, 1.5 mL, 10mM dNTPs mixture solution 1ml
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Inquire
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