Enzyme Properties
5' to 3' exonuclease activity: No
3' to 5' exonuclease activity: No
3’ – A Overhang: No
Strand Displacement: Yes Fragment Size: Up to 9 kb
Application
First strand cDNA synthesis from RNA, RT-PCR, and qRT-PCR
Reagents Supplied
5 x Reaction Buffer: 50 mM Tris-HCl, 250 mM KCl, 10 mM MgCl2, pH 8.1
100 mM DTT
dNTP Mix: 2.5 mM of each dNTP
Concentration
200 U/μl
Storage Conditions
50% glycerol containing 20 mM Tris-Cl (pH7.6), 150 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1 % IGEPAL CA-630
Store Temperature
-20°C
Unit Definition
One unit is defined as the amount of enzyme required to incorporates 1 nmole of dTTP into acid-precipitable material in 10 minutes at 37°C using poly (A)oligo (dT) as template primer.
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Manual
• M-MLV Reverse Transcriptase
MSDS
• M-MLV Rtase
• M-MLV Rtase dilution buffer
• M-MLV reaction buffer
• 100mM DTT
Brochure
• Enzymes 2010 Brochure
Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
• ISO 9001:2008 - certificate
• EN ISO 13485:2003 / AC:2009 - certificate
• EC Directive 98/79/EC - certificate
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