M-MLV Reverse Transcriptase


For cDNA Synthesis, RT-PCR, and qRT-PCR

Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase is an RNA-dependent DNA polymerase. This enzyme is able to use an RNA molecule as a template and synthesize a double-stranded DNA. M-MLV Reverse Transcriptase is isolated from an E.coli strain containing a recombinant clone. It is ideal for use of first-strand synthesis cDNA from RNA molecules and for cDNA synthesis for Reverse Transcriptase PCR and qRT-PCR

Features and Benefits

  • Optimized 5X buffer: For more full length fragments - within 10 minutes
  • Full length cDNA: For genes up to 9 kb
  • RNase, DNase and Proteinase-free: Ensures the integrity of your samples
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Enzyme Properties

5' to 3' exonuclease activity: No
3' to 5' exonuclease activity: No
3’ – A Overhang: No
Strand Displacement: Yes Fragment Size: Up to 9 kb

 

Application

First strand cDNA synthesis from RNA, RT-PCR, and qRT-PCR

 

Reagents Supplied

5 x Reaction Buffer: 50 mM Tris-HCl, 250 mM KCl, 10 mM MgCl2, pH 8.1
100 mM DTT
dNTP Mix: 2.5 mM of each dNTP

 

Concentration

200 U/μl

 

Storage Conditions

50% glycerol containing 20 mM Tris-Cl (pH7.6), 150 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1 % IGEPAL CA-630

 

Store Temperature

-20°C

 

Unit Definition

One unit is defined as the amount of enzyme required to incorporates 1 nmole of dTTP into acid-precipitable material in 10 minutes at 37°C using poly (A)oligo (dT) as template primer.

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Manual

M-MLV Reverse Transcriptase


MSDS

M-MLV Rtase

M-MLV Rtase dilution buffer

M-MLV reaction buffer

100mM DTT


Brochure

Enzymes 2010 Brochure


Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

ISO 9001:2008 - certificate

EN ISO 13485:2003 / AC:2009 - certificate

EC Directive 98/79/EC - certificate

 

 

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