T7 RNA Polymerase is isolated from E.coli cells containing the ligase gene cloned from T7 bacteriophage.
T7 RNA Polymerase is a DNA dependent RNA polymerase with a highly specificity for the initiation of transcription at T7 RNA polymerase promoters. It is widely used for the rapid synthesis in vitro of specific RNAs.
Features and Benefits
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Source: T7 RNA Polymerase is isolated from E.coli cells containing the ligase gene cloned from T7 bacteriophage.
Applications
- Synthesis of RNA transcripts for northern hybridization and southern hybridization probes.
- RNA generation for studies of RNA structure, procession and catalysis
Supplied with Enzyme
- 10 x Reaction Buffer (1 ml): 400 mM Tris-HCl (pH 8.0), 60 mM MgCl2, 20 mM Spermidine
- 100 mM DTT (0.5 ml)
- DEPC-DW (1ml)
Storage Condition
200 mM Na-phosphate (pH 7.7), 10 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.02 % Triton x -100, 0.08% Tween-20, 50% Glycerol, Store at -20°C
Concentration
20.1 units/㎕
Unit Definition
One unit of enzyme catalyzed incorporation of 1 nmole of [3H]ATPs into acid insoluble form in 60 min at 37°C
Quality Assurance
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Nuclease Contamination Assay |
No altered was detected after incubation of 1 ug of substrate DNA with 500 units of T7 RNA polymerase for 18 hrs in 37°C |
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Protease Contamination Assay |
No altered pattern was detected after in cubation of 2,000 units of T7 RNA polymerase for 18 hrs in 37°C |
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Cat.No.
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Product Description
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Price
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Add to Cart
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| E-3041
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T7 RNA Polymerase, 2,000 U
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Inquire
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| E-3042
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T7 RNA Polymerase, 10,000 U(5 x 2,000 U)
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Inquire
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