AccuPower® PCR PreMix(with UDG) is a ready-to-use master mix for high-specificity DNA amplification, con-taining UDG to prevent carryover contamination or crossover contamination
The polymerase chain reaction (PCR) can amplify a single molecule over a billionfold. Thus, even minuscule amounts of a contaminant can be amplified and lead to a false positive result. Such contaminants are often products from previous PCR amplifications (carry-over contamination). Therefore, researchers have developed methods to avoid such contamination AccuPower® PCR PreMix(with UDG) is a ready-to-use master mix containing all components, except primers, for the amplification and detection of DNA in PCR The master mix combines Top DNA polymerase with integrated UDG carryover prevention technology to provide optimal performance with a variety of PCR detection technologies
Features and Benefits
Prevention of carryover contamination
UDG and dUTP in the MasterMix prevent the reamplification of carryover PCR products between reactions. dUTP ensures that any amplified DNA will contain uracil, while UDG removes uracil residues from single- or double-stranded DNA, preventing dU-containing DNA from serving as template in future PCRs, A UDG incubation step(37°C,2min) before PCR cycling destroys any contaminating dU-containing product from previous reactions. UDG is then inactivated by the high temperatures during normal PCR cycling, thereby allowing the amplification of genuine target sequences
Easy-to-use
All reaction components required for PCR, including thermostable DNA polymerase and dNTPs are contained within each tube and in a lyophilized "PreMix" form. The user needs only to add template DNA, primers and water. Materials necessary for loading agarose gels for electrophoresis are also added in the reaction, negating the need to add loading dye after PCR is completed
Reproducibility
Each batch is produced under strict quality controls. Errors that commonly occur during mass production are eliminated during the individual packaging process. Bioneer’s current batch processing system allows for the production of more accurate and reproducible end-product yield
Specifications
5' to 3' exonuclease: No
3' to 5' exonuclease: No
3’ – A Overhang: Yes
Fragment Size: ~ 10 kb
Application
Polymerase chin reaction (PCR)
Primer extension
TA cloning
Gene sequencing
Molecular Diagnosis
Experimental data
Sensitivity
Figure 1. Comparison of sensitivity between Accupower® PCR PreMix and Accupower® PCR PreMix(with UDG).
Sensitivity test was operated using serial diluted human genomic DNA. Reaction mixture was incubated at 37°C for 2min followed by 95°C for 5min, 30 cycles of 20sec at 95°C, 20sec at 55°C, 30sec at 72°C. Accupower® PCR PreMix(with UDG) contains dUTP besides dATP, dGTP, dCTP and dTTP. The amount of DNA used to test is represented below.
Lane 1: 10ng of Human genomic DNA Lane 2: 1ng of Human genomic DNA
Lane 3: 100pg of Human genomic DNA Lane 4: 10pg of Human genomic DNA
M: 100 bp DNA ladder (Bioneer Cat. No: D-1030-1)
Figure 2. Comparison of amplification quality using PCR products(not including uracil base) between Accupower® PCR PreMix and Accupower® PCR PreMix(with UDG).
Amplification quality test was operated using serial diluted PCR products not including uracil base.
Accupower® PCR PreMix was also tested for negative control. Reaction mixture was incubated at 37°C for 2min, followed by 95°C for 5min, 30 cycles of 20sec at 95°C, 20sec at 55°C, 30sec at 72°C.
Accupower® PCR PreMix(with UDG) contains dUTP besides dATP, dGTP, dCTP and dTTP. The copy number of PCR products(copy) used to test is represented below.
Lane 1: 10
11copy Lane 2: 10
10copy Lane 3: 10
9copy
Lane 4: 10
8copy Lane 5: 10
7copy Lane 6: 10
6copy
Lane 7: 10
5copy Lane 8: 10
4copy Lane 9: 10
3copy
Lane 10: 10
2copy Lane N: No template control
M: 100 bp DNA ladder (Bioneer Cat. No: D-1030-1)
Prevention of carryover contamination
Figure 3. Efficiency of uracil DNA glycosylase using PCR products(including uracil base).
Efficiency test of uracil DNA glycosylase was operated using serial diluted PCR products including uracil base.
Accupower® PCR PreMix was also tested for negative control.
Reaction mixture was incubated at 37°C for 2min, followed by 95°C for 5min, 30 cycles of 20sec at 95°C, 20sec at 55°C, 30sec at 72°C.
Accupower® PCR PreMix(with UDG) contains dUTP besides dATP, dGTP, dCTP and dTTP.
The copy number of PCR products used to test is represented below.
Lane 1: 10
11copy Lane 2: 10
10copy Lane 3: 10
9copy
Lane 4: 10
8copy Lane 5: 10
7copy Lane 6: 10
6copy
Lane 7: 10
5copy Lane 8: 10
4copy Lane 9: 10
3copy
Lane 10: 10
2copy Lane N : No template control M: 100 bp DNA ladder (Bioneer Cat. No: D-1030-1)
Manual
• AccuPower® PCR PreMix(with UDG)
MSDS
• MSDS_AccuPower® PCR PreMix(with UDG)
Brochure
• AccuPower® PreMix series - 2010 Brochure
Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
• ISO 9001:2008 - certificate
• EN ISO 13485:2003 / AC:2009 - certificate
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Cat.No.
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Product Description
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Price
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Add to Cart
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| K-2012-1
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AccuPower® PCR PreMix (with UDG), 0.2 ml thin-wall 8-strip tubes with attached cap / 96 tubes
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Inquire
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| K-2016-1
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AccuPower® PCR PreMix (with UDG), 0.2 ml thin-wall 8-strip tubes with attached cap / 480 tubes
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Inquire
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