25/100 bp Mixed DNA Ladder

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Ready-to-load 25/100 bp Mixed DNA Ladder ranging from 25 to 2,000 bp. Wide range of DNA Ladders and Molecular Weight Markers

25/100 bp Mixed DNA Ladder is designed for determining the size of double stranded DNA from 25 to 2,000 bp. The ladder consists of 17 double stranded DNA fragments ranging in size from 25 to 200 bp in 25 bp increments, from 200 to 1,000 bp in 100 bp increments, and an additional fragment of 2,000 bp. The DNA marker fragments at 150, 500, 1,000 and 2,000 bp bands are two times brighter for easy identification.

Note

The DNA ladder can be applied directly onto an agarose gel. There is no need to heat before loading. Repeated freezing and thawing should be avoided.

Features and Benefits

Complete and ready to load: Convenient

Easy-to-Identify reference bands: Easy-to-read results

Competitive pricing: Great value for your research dollar

Specifications

Concentration: 150 ng/μl
Recommended loading: 2.0 μl / 5 mm lane width
Typical Number of lanes: 125 (5 mm lane width)
Size range (bp): 25 - 2,000
Number of bands: 17
Supplied in: 10 mM Tris-HCl (pH 8.0), 1 mM EDTA, 2.5% Ficoll, 0.005% Bromphenol Blue, 0.005% Xylene Cyanol
Storage: -20°C

2.5% TBE agarose gel stained with Ethidium Bromide.

Nuclease Activity Test

Lane 1, 2, 3, 4: 25/100 bp Mixed DNA Ladder was stored at 4°C for 16 hours and volume loaded at 2 μl, 3 μl, 5 μl, and 6 μl.
Lane 5, 6, 7, 8: 25/100 bp Mixed DNA Ladder was stored at 37°C for 16 hours and volume loaded at 2 μl, 3 μl, 5 μl, and 6 μl.
2.5% TBE agarose gel was stained with Ethidium Bromide.