ExiProgen™ His-tagged Protein Purification Kit

OverviewTechnical/SpecsLiterature/SupportOrdering Information

Fully automatic micro-scale purification of His-tagged protein

ExiProgen™ His-tagged Protein Purification kit is suitable to extract of Histidine-tagged protein from recombinant E. coli, by automatic instrument ExiProgen™.
Ni-NTA Magnetic Silica Resins are silica beads, with an average diameter of 1~5um, that contain magnetic particles and have strongly metal-chelating nitrilotriacetic acid (NTA) groups covalently bound to their surfaces.

Features and Benefits

Automatic purification of His-tagged proteins

Getting highly pure proteins through SSMB(Spherical Shape Magentic Beads)

Reproducible result by minimizing human handling

Finishing whole process within 30 minutes

Application

Figure 1. Reproducibility test: ExiProgen™ His-tagged Protein Purification Kit provides reproducible results, the first time and every time. Electrophoresis was performed on a vertical gel containing 13% poly-acrylamide.
Lane M: AccuLadder™ Protein Size Marker
Lane 1: crude extract of gram negative cell by sonication
Lane 2-3: unbound protein (replicates)
Lane 4, 5, 6, 7, 8, 9, 10, 11: Purified PTPase #32
- Sample type : Recombinant E.coli cell
- Starting volume : 500 uL
- Elution volume : 250 uL
- Average yield : 200 ug/well
- Average purity : 80%

Figure 2. Flexibility test: Proteins as small as 10 kD and as large as 120 kD have been purified with the ExiProgen™ His-tagged Protein Purification Kit. Electrophoresis of purified diverse size of proteins by ExiProgen™ His-tagged Protein Purification Kit was performed on a vertical gel containing 10% poly-acrylamide.
Lane M: AccuLadder™ Protein Size Marker (Broad)
Lane 1: PTPase #1 (65kD), Lane 2: Polymerase (95kD),
Lane 3: PTPase #11 (36kD), Lane 4: PTPase #37 (18kD),
Lane 5: PTPase #32 (23kD), Lane 6: PTPase #39 (19kD);
- Sample type : Recombinant E.coli cell
- Starting volume : 500 uL
- Elution volume : 250 uL
- Average yield : 200 ug/well
- Average purity : 80%

Procedure