AccuPower® RT-PCR PreMix

cDNA synthesis followed by PCR in one tube



AccuPower® RT-PCR PreMix contains all the components necessary for sequential cDNA synthesis and amplification in one tube (one-step RT-PCR). This RT-PCR PreMix consists of both M-MLV Reverse Transcriptase, RNA dependent DNA polymerase, and a thermostable DNA polymerase in a lyophilized mix of dNTPs, reaction buffer, RNase inhibitor, tracking dye, and stabilizer. The kit can be used for double stranded cDNA synthesis from low copy RNA or mRNA and subsequent RT-PCR .

Features and Benefits

• Convenient lyophilized RT: Easy to use, simply add your purified RNA
•  High yield of cDNA: For genes up to 6 kb
•  Stable for 2 years at -20°C: Long life
•  RNase, DNase and Proteinase-free: Ensures the integrity of your samples
   

Specifications

5' to 3' exonuclease: No
3' to 5' exonuclease: No
3’ – A Overhang: No
Fragment size: 6 kb

 

Application

cDNA synthesis followed by PCR

Figure 1. Specific amplification of 5' - UTR region of HCV with AccuPower® RT-PCR PreMix.
Human DNA was amplified to generate 0.1-1.5 kb PCR fragments using 1 U of polymerase. The comparison was performed under the same experimental conditions.

Lane 1: DNA Ladder (BM corp.)
Lane 2: Negative control
Lane 3, 4: HCV positive serum
Lane 5: HCV negative serum
 

Figure 2. Sensitivity comparison between AccuPower® RT/PCR PreMix and Supplier I RT-PCR Kit
Each 10⁹ copy – 10³ copy of PSTVd used for RT/PCR and the same amount of RT/PCR products used for electrophoresis

Lane1: 10⁹ copy Lane2: 10⁸ copy
Lane3: 10⁷ copy Lane4: 10⁶ copy
Lane5: 10⁵ copy Lane6: 10⁴ copy
Lane7: 10³ copy Lane8: NTC
 

Figure 3. Comparison of reproducibility test for AccuPower RT/PCR PreMix batch 1,2,3 and batch 4 products using serial diluted Human Total RNA.
Each 10⁹ copy – 10³ copy of PSTVd used for RT/PCR and the same amount of RT/PCR products used for electrophoresis

Lane 1: 10 ng Human total RNA from HeLa cell
Lane 2: 1 ng Human total RNA from HeLa cell
Lane 3: 100 pg Human total RNA from HeLa cell
Lane 4: 10 pg Human total RNA from HeLa cell

Manual
• AccuPower® RT-PCR PreMix

MSDS
• AccuPower® PCR Master Mixes

Brochure
• AccuPower® PCR Master Mixes - 2010 Brochure

Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001:2008 - certificate

• EN ISO 13485:2003 / AC:2009 - certificate

Cat.No.	Product Description	Price	Add to Cart
K-2055	AccuPower® RT/PCR PreMix, 0.2 ml thin-wall 8-strip tubes with attached cap / 96 tubes, 20 ul reaction	Inquire
K-2055-B	AccuPower® RT/PCR PreMix, 0.2 ml thin-wall 8-strip tubes with attached cap / 480 tubes, 20 ul reaction	Inquire
K-2056	AccuPower® RT/PCR PreMix, 0.5 ml thin-wall tubes with attached cap / 100 tubes, 50 ul reaction	Inquire
K-2057	AccuPower® RT/PCR PreMix, 0.2 ml thin-wall 8-strip tubes with attached cap / 96 tubes, 50 ul reaction	Inquire
K-2057-B	AccuPower® RT/PCR PreMix, 0.2 ml thin-wall 8-strip tubes with attached cap / 480 tubes, 50 ul reaction	Inquire