1 kb DNA Ladder

OverviewTechnical/SpecsLiterature/SupportOrdering Information

Ready-to-load 1 kb DNA Ladder ranging from 500 to 10,000 bp.
Wide range of DNA Ladders and Molecular Weight Markers

1 kb DNA Ladder is designed for determining the size of double stranded DNA ranging between 500 to 10,000 bp. The 1 kb Ladder consists of DNA fragments 0.5, 1.0, 1.6, 2.0, 3 (2.961), 4, 5 (5.007), 6 (5.991), 8 and 10 (10.2) kb. The 2.961 kb band is approximately two times brighter for easy identification.

Note

The DNA ladder can be applied directly onto an agarose gel. There is no need to heat before loading. Repeated freezing and thawing should be avoided.

Features and Benefits

Complete and ready to load: Convenient

Easy-to-Identify reference bands: Easy-to-read results

Competitive pricing: Great value for your research dollar

Specifications

Concentration: 130 ng/μl
Recommended loading: 2.0 μl / 5 mm lane width
Typical Number of lanes: 250 (5 mm lane width)
Size range (bp): 500 - 10,200
Number of bands: 10
Supplied in: 10 mM Tris-HCl (pH 8.0), 1 mM EDTA, 2.5% Ficoll, 0.005% Bromphenol Blue, 0.005% Xylene Cyanol
Storage: -20°C

1.0% TAE agarose gel stained with Ethidium Bromide.

Nuclease Activity Test

Lane 1, 2, 3: 1 kb DNA Ladder was stored at 4°C for 16 hours and volume loaded at 2 μl, 3 μl, and 5 μl.
Lane 4, 5, 6: 1 kb DNA Ladder was stored at 37°C for 16 hours and volume loaded at 2 μl, 3 μl, and 5 μl.
1.0% TAE agarose gel was stained with Ethidium Bromide.