ExiProgen™ Bacteria Genomic DNA Kit

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For various cultured bacterial cells, Automatic Genomic DNA Extraction Kit – ExiProgen™ Bacteria Genomic DNA Extraction Kit

ExiProgen™ Bacteria Genomic DNA Kit
ExiProgen™ Bacteria Genomic DNA utilizes a resuspension buffer that efficiently disrupts cells and proteins for efficient extraction of genomic DNA. Gram (+) bacteria needs specific pre-treatment before the re-suspension step using Lyticase or Lysozyme (not included). The binding buffer and silica magnetic particles will bind genomic DNA to the surface of the beads. The reaction containers are then subject to a magnetic field, forcing the genomic DNA-bound silica magnetic particles to remain within the wells while the reaction mixture and cellular waste are removed. Three types of washing buffers are introduced sequentially during in the next step to remove the remainder of cellular waste products. The washing buffers are then pipetted off, while the genomic DNA-bound silica magnetic particles remain bound by the magnetic field. DNase-free elution buffer is then introduced to the silica magnetic particles to release the bound and now purified genomic DNA.

Features and Benefits

Reproducibility & Reliability

ExiProgen™ Bacteria Genomic DNA Kit functions through a buffer cartridge system containing buffers optimized for the extraction of genomic DNA from Gram (-) Bacteria and Gram (+) Bacteria samples. Nucleic acid extractions occur with the buffer cartridges through the automated nucleic acid extraction instrument, allowing for reproducible performance. Also, by preventing human errors during the workflow, the results are always dependable. The product is produced under ISO 9001 quality standards by an automated production facility to provide you with high-quality performance.

High-Yield and Purity

The buffer composition within the ExiProgen™ Bacteria Genomic DNA Kit was developed to extract high purity and high yield genomic DNA from Gram (-) Bacteria and Gram (+) Bacteria samples. Also, the extraction protocols contained within the automated nucleic acid extraction instruments ExiProgen™ is optimized for maximum performance. Anyony can extract genomic DNA of high purity and yield from Gram (-) Bacteria and Gram (+) Bacteria samples by using the pre-programmed and optimized protocols in ExiProgen™.

Contents

Components	Quantity	Note
Buffer Cartridge ①	6 ea
Buffer Cartridge ②	6 ea
Resuspension buffer	1 ea	25 ml
Disposable filter tip	3 pack	32 ea/pack
Elution tube	1 pack	12 ea/pack
Manual	1 ea

Specifications

Starting culture volume	up to 1X10⁹ cells
Elution volume	50 - 100 ul
Expected Yield	up to 10 ug
Expected Purity (A260/280)	> 1.8

Applications

PCR, Quantitative real time PCR, Gene cloning, Genetics, Classification of bacteria

Procedure

Experimental Data

Fig. 1 Agarose gel electrophoresis results of genomic DNA extracted from E.coli cell(1X10⁹ cells)
Results of gel electrophoresis of genomic DNA extracted from E.coli cell(1X10⁹ cells). Yields were on average 8-12ug and purities (A₂₆₀/A₂₈₀) were at least (average) 1.8. Also, to observe cross-contamination that may occur during the extraction process, the E.coli cell(1X10⁹ cells) were placed in a checkerboard pattern, and the rest of the wells were filled with D.W. No cross-contamination could be detected. M; Size marker, S; Extraction with E.coli cell(1X10⁹ cells) sample, B; Extraction with D.W only.

Fig. 2 Real-time PCR results(MTB & NTM) of genomic DNA extracted from sputum samples
Real-time PCR results using an AccuPower® MTB & NTM EX Real-Time PCR Kit(MTN-1111, Bioneer) and run on Exicycler™ 96 Real-Time Quantitative Thermal Block (A-2060, Bioneer) from genomic DNA extracted from 10ml of sputum samples.