Predesigned and validated Real-Time PCR primers for measuring siRNA knockdown results
The AccuTarget™ Human Validated Real-Time PCR Primer Library is comprised of highly specific and sensitive Real-Time primer sets that are bioinformatically designed and validated based on the human genome. The Real-Time PCR Primer Library consists of 11,154 primer sets validated in Real-Time PCR and used to measure siRNA knockdown results
Features and Benefits
-
In ready-to-ship format: 11,154 human genes specific primers
-
All Primers pre-validated: QC tested via MALDI-TOF mass spectrometer
-
Competitive pricing: Great value for your research dollar
The
AccuTarget™ Human Validated Real-Time PCR Primer Library is comprised of highly specific and sensitive Real-Time primer sets that are bioinformatically designed and validated based on the human genome. The Real-Time PCR Primer Library consists of 11,154 primer sets validated in Real-Time PCR with SYBR Green detection using
Exicycler™ 96 and
AccuPower GreenStar™ qPCR PreMix. The Library is categorized by gene function and pathway. The Library guarantees the most specific and sensitive Real-Time PCR result when used with
AccuPower GreenStar PCR PreMix (Not available in the US).
Figure 1. Real-Time PCR validation test of Human Oxidoreductase using
AccuTarget™ Human Oxidoreductase Real-Time PCR Primer Set (A; Amplification curve, B; Melting curve analysis) Real-Time PCR reaction for
AccuTarget™ Human Oxidoreductase Real-Time PCR Primer Set was tested using Exicycler 96 Real-Time Quantitative Thermal Block (Cat. No. A-2060) and
AccuPower GreenStar PCR PreMix (Cat. No. K-6210). cDNA template was reverse-transcribed from QPCR Human Reference Total RNA 30 ng and reaction condition was 95°C 10 minutes, 1 cycle and 95°C 10 seconds, 58°C 25 seconds, 72°C 30 seconds, 41 cycles.
Figure 2. Real-Time PCR validation test of hHPRT1 and hSLC12A1 genes (A; Amplification curve of hHPRT1, B; Melting curve analysis of hHPRT1, C; Amplification curve of hSLC12A1, D; Melting curve analysis of hSLC12A1) Real-Time PCR for hHPRT1 Primer Set and hSLC12A1 Primer Set were tested using Exicycler 96 Real-Time Quantitative Thermal Block (Cat. No. A-2060) and
AccuPower GreenStar PCR PreMix (Cat. No. K-6210). cDNA template was reverse-transcribed from qPCR Human Reference Total RNA 30 ng (60 ng) and reaction condition was 95°C 10 minutes, 1 cycle and 95°C 10 seconds, 58°C 25 seconds, 72°C 30 seconds, 41 cycles.
Figure 3. Real-Time PCR sensitivity test for detection of hPGK1 (Phosphoglycerate Kinase 1; Housekeeping gene) gene using
AccuTarget™ hPGK1 primer set. (A; Amplification curve, B; Standard curve analysis, C; Melting curve analysis, 1; 100 ng QPCR Human Reference Total RNA, 2; 10 ng QPCR Human Reference Total RNA, 3; 1 ng QPCR Human Reference Total RNA, 4; 100 pg QPCR Human Reference Total RNA, 5; 10 pg QPCR Human Reference Total RNA, N; NTC [No Template Control]) Real-Time PCR reaction for hPGK1 primer set was tested using Exicycler 96 Quantitative Real-Time Thermal Block (Cat. No. A-2060),
AccuPower GreenStar PCR PreMix (Cat. No. K-6210) and Opticon (BioRad). cDNA template was reverse-transcribed from QPCR Human Reference Total RNA 400ng (serial dilution; 100 ng, 10 ng, 100 pg, 10 pg) and reaction condition was 95°C 10 minutes, 1 cycle and 95°C 10 seconds, 58°C 25 seconds, 72°C 30 seconds, 41 cycles.
Figure 4. Comparison of Real-Time PCR reproducibility test of hPGK1 (Phosphoglycerate Kinase 1; Housekeeping gene) gene detecting primer set from Bioneer and competitors. [A, B & C:
AccuTarget™ hPGK1 Primer Set, D, E & F; Competitor Q hPGK1 primer set, A & D; Amplification curve, B & E; Standard curve analysis, C & F: Melting curve analysis, 1; 100 ng QPCR Human Reference Total RNA, 2; 10 ng QPCR Human Reference Total RNA, 3; 1 ng QPCR Human Reference Total RNA, 4; 100 pg QPCR Human Reference Total RNA, 5; 10 pg QPCR Human Reference Total RNA, N; NTC (No Template Control)] Real-Time PCR reaction for hPGK1Primer Set was tested using Opticon (BioRad) and
AccuPower GreenStar qPCR PreMix (Cat. No. K-6210). cDNA template was reverse-transcribed from QPCR Human Reference Total RNA 400ng (serial dilution; 100 ng, 10 ng, 100 pg, 10 pg) and reaction condition was 95°C 10 minutes, 1 cycle and 95°C 10 seconds, 58°C 25 seconds, 72°C 30 seconds, 41 cycles.
Figure 5. QC test for
AccuTarget™ Validated Real-Time PCR primer Library for Human Quality control of Real-Time PCR primer Library was tested using MALDI-TOF instrument that analyzes oligo mass.