AccuPower® RocketScript™ RT PreMix

RocketScript™ the ONLY M-MLV Reverse Transcriptase Thermostable up to 70°C

AccuPower® RocketScript™ RT PreMix contains BIoneer’s exclusive M-MLV based thermostable reverse transcriptase (RTase), RocketScript™. Native M-MLV RTase has maximum activity at relatively low temperatures (42°C), causing several problems in reverse transcription of RNA molecules with complex secondary structure.

RocketScript™ has thermostable activity (42°C~70°C), allowing efficient cDNA synthesis from virtually any RNA. The lyophilized PreMix contains all components necessary for a successful reverse transcription reaction, including RTase, RNase inhibitor and buffer components. Just add template RNA, primers and water, and the RT reaction is ready to go.

Schematic representation of the 5’UTR of a gene, with complex secondary structure, at three different temperatures. Note that RocketScript™ shows full activity at 70°C allowing it to synthesize the complete gene sequence where M-MLV and other Reverse Transcriptase's fail.
 

Features and Benefits

• Thermostable Activity: RocketScript™ is able to perform reverse transcription reactions throughout a wide range of temperatures from 42°C to 70°C.

• Enhanced Performance: RocketScript™ has enhanced performance to handle both high and low input RNA concentrations as well as short and long RT target sizes.

• Easy-to-use: The product contains the enzyme itself, plus RNase inhibitors and all other components necessary for the best reverse transcription results in the tube.

• Reproducibility: Bioneer's strict quality controlled production system ensures that your results will be reproducible experiment after experiment.

• Convenient: Just add template and primers and start your reaction. dNTPs, buffer and enzyme are provided.

• Stability: Stable at room temperature for a month one year at 4°C and for 2 years in a -20°C freezer

• RNase, DNase and Proteinase-free: Ensures the integrity of your samples.

• Broad range of working temperatures: For RNAs that are G:C rich or significant secondary structure

• Stable for 2 years at -20°C: Long shelf life

Specifications

5' to 3' exonuclease: No
3' to 5' exonuclease: No
3’ – A Overhang: No
Fragment Size: Up to 10 kb

 

Application

- First-strand synthesis of cDNA from RNA molecules (RT)
- RT-PCR
- Random priming reaction
- Library construction
- Probe labeling
- mRNA 5end Mapping by Primer Extension Analysis
- Real time PCR

 

Experimental data

Figure 1. Sensitivity comparison between AccuPower® RocketScript™ RT PreMix and M-MLV RTase
Sensitivity results of AccuPower® RocketScript™ RT PreMix using GAPDH compared with conventional Reverse transcriptases.
Each 100 ng – 10 fg of total RNA used for RT and the same amount of RT products used for electrophoresis.
Lane 1: 10 fg Human total RNA from HeLa cell Lane 2: 100 fg Human total RNA from HeLa cell
Lane 3: 1 pg Human total RNA from HeLa cell Lane 4: 10 pg Human total RNA from HeLa cell
Lane 5: 100 pg Human total RNA from HeLa cell Lane 6: 1 ng Human total RNA from HeLa cell
Lane 7: 10 ng Human total RNA from HeLa cell Lane 8: 100 ng Human total RNA from HeLa cell


Figure 2. Comparison of amplification efficiency between AccuPower® RocketScript™ RT PreMix and competitors M-MLV RTase.  
    RocketScript™ is able to handle a wide range of sample concentrations and transcript lengths so your downstream applications are minimally effected by the reverse transcription step.
Lanes 1-3: 1,000 ng, 100 ng and 10 ng of total RNA from HeLa cells, respectively.

Figure 3. Sensitivity comparison between AccuPower® RocketScript™ RT PreMix and competitor RTases using Real Time PCR
Reverse transcription conditions: conventional 1 hr incubation at 60°C, deactivation at 95°C for 5 min All cDNAs were amplified with AccuPower® DualStar™ qPCR PreMix (K-6110) from Bioneer.

Concentration	RocketScript™ RT PreMix	Supplier Q	Supplier I
10,000	23.91	25.63	24.43
1,000	27.33	28.92	28.03
100	30.62	32.42	30.88
10	33.63	35.43	33.95
Efficiency	104%	103%	108%
Linearity	0.99999	0.9996	0.9995

 

 

 

Manual
• AccuPower® RocketScript RT PreMix

MSDS
• AccuPower® RocketScript RT PreMix

Brochure
• AccuPower® RocketScript RT PreMix - 2011 Brochure

Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001:2008 - certificate

• EN ISO 13485:2003 / AC:2009 - certificate

Cat.No.	Product Description	Price	Add to Cart
K-2101	AccuPower® RocketScript™ RT Premix, 0.2ml thin-wall 8-Strip tubes with attached cap/96tubes, 20ul/Rxn	Inquire
K-2102	AccuPower® RocketScript™ RT Premix, 0.2ml thin-wall 8-Strip tubes with attached cap/480tubes, 20ul/Rxn	Inquire
K-2103	AccuPower® RocketScript™ RT Premix, 0.2ml thin-wall 8-Strip tubes with attached cap/96tubes, 50ul/Rxn	Inquire
K-2104	AccuPower® RocketScript™ RT Premix, 0.2ml thin-wall 8-Strip tubes with attached cap/480tubes, 50ul/Rxn	Inquire