ExiProgen™ Tissue total RNA Kit

For various animal tissue samples, Automatic total RNA Extraction Kit – ExiProgen™ Tissue total RNA Kit



ExiProgen™ Tissue total RNA Kit
ExiProgen™ Tissue total RNA Kit uses Tissue lysis buffer ① and Tissue lysis buffer ② contained within the product to efficiently disrupt animal tissues and proteins for efficient extraction of total RNA without degradation. The binding buffer and silica magnetic particles will bind total RNA to the surface of the beads. The reaction containers are then subject to a magnetic field, forcing the total RNA-bound silica magnetic particles to remain within the container while the reaction mixture and cellular waste are removed. Three types of washing buffers +are introduced sequentially during in the next step to remove the remainder of cellular waste products. The washing buffers are then pipetted off, while the total RNA-bound silica magnetic particles remain bound by the magnetic field. RNase-free elution buffer is then introduced to the silica magnetic particles to release the bound and now purified total RNA.

Features and Benefits

• Reproducibility & Reliability

ExiProgen™ Tissue Total RNA Kit functions through a buffer cartridge system containing buffers optimized for the extraction of total RNA from various animal tissue samples. Nucleic acid extractions occur with the buffer cartridges through the automated nucleic acid extraction instrument, allowing for reproducible performance. Also, by preventing human errors during the workflow, the results are always dependable.
The product is produced under ISO 9001 quality standards by an automated production facility to provide you with high-quality performance.

• High-Yield and Purity

The buffer composition within the ExiProgen™ Tissue Total RNA Kit was developed to extract high purity and high yield total RNA from various animal tissue samples. Also, the extraction protocols contained within the automated nucleic acid extraction instruments ExiProgen™ is optimized for maximum performance. Anybody can extract total RNA of high purity and yield from tissue samples by using the pre-programmed and optimized protocols in ExiProgen™.

 

Contents

Specifications

 

Applications

RT-PCR, Quantitative real time RT-PCR, cDNA synthesis, Chip-array analysis

 

Procedure




Experimental Data



Fig. 1 Electrophoresis result with LabChip GX
Results of capillary electrophoresis of total RNA extracted from tissue sample(rat liver, 15mg) using LabChip GX(Caliper life science, USA). Average RQS(RNA Quality Score) were at least 9.4.



Fig. 2 Real-time RT-PCR results of total RNA extracted from animal tissue
Real-time RT-PCR results using an AccuPower® RocketScrpt™ RT PreMix(K-2101, Bioneer)와 AccuPower® Dualstar™ qPCR PreMix(K-6110, Bioneer) and run on ExicyclerTM 96 Real-Time Quantitative Thermal Block (A-2060, Bioneer) from total RNA extracted from liver sample (rat, 15mg).




 

Application Note

• ExiProgen™ Tissue total RNA Kit_Rat liver

Manual

• ExiProgen™ Tissue total RNA Kit

MSDS

• ExiProgen™ Tissue total RNA Kit

Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001:2008 - certificate

Cat.No.	Product Description	Price	Add to Cart
K-4342	ExiProgen™ Tissue total RNA Kit, 96 reactions	Inquire