AccuPower® Multiplex PCR PreMix can simultaneously detect one or more products in a one tube

Features and Benefits

• Fexibility: Up to 20 different target genes from human genomic DNA can be amplified in a single tube
• Easy-to-use:  All reaction components required for PCR, including thermostable DNA polymerase and dNTPs are contained within each tube and in a lyophilized “PreMix” form. The user needs only to add template DNA, primers and water to perform up to 20-plex PCR. Materials necessary for agarose gel electrophoresis are also added in the reaction, negating the need to prepare the samples for electrophoresis after PCR is completed.
• Reproducibility: Each batch is produced under strict quality controls. Errors that commonly occur during mass production are eliminated during the individual packaging process. Bioneer’s current batch processing system allows for the production of more accurate and reproducible end-product yield.

Specifications

5' to 3' exonuclease: No
3' to 5' exonuclease: No
3’ – A Overhang: Yes
Fragment Size: ~1 kb

Application
 

Figure 1. Single PCR and Multiplex PCR using AccuPower® Multiplex PCR PreMix

Each lane from left to right indicates the single and Multiplex PCR product using AccuPower® Multiplex PCR PreMix.
a) 10plex Multiplex PCR, b) 20plex Multiplex PCR
M; 25/100 bp Mixed DNA Ladder (cat. No. D-1020)


Figure 2. Comparison of amplification quality between AccuPower® Multiplex PCR PreMix and other supplier’s Multiplex PCR kit.

6-plex primers were added into AccuPower® Multiplex PCR PreMix and other supplier’s Multiplex PCR kit. A series of Human genomic DNA diluents were tested. ( Lane 1. Human genomic DNA 100ng, Lane 2. Human genomic DNA 10ng, Lane 3. Human genomic DNA 1ng ). All data were obtained using Mygene 96 machine (Applied Bioneer co.)
Supplier Q : Q company Multiplex PCR kit, Supplier S : S company Multiplex PCR kit, Supplier I : I company Taq polymerase for Mutiplex PCR (0.5U, added 2mM MgCl₂) Rxn. Condition : 95°C for 10min, followed by 35cycles of 30sec at 95°C, 30sec at 60°C, 60sec at 72°C M; 25/100 bp Mixed DNA Ladder (cat. No. D-1020)

Figure 3. Comparison of amplification quality between AccuPower® Multiplex PCR PreMix and other supplier’s Multiplex PCR kit.

10-plex primers were added into AccuPower® Multiplex PCR PreMix and other supplier’s Multiplex PCR kit. A series of Human genomic DNA diluents were tested. ( Lane 1. Human genomic DNA 100ng, Lane 2. Human genomic DNA 10ng, Lane 3. Human genomic DNA 1ng ). All data were obtained using Mygene 96 machine (Applied Bioneer co.)
Supplier Q : Q company Multiplex PCR kit, Supplier S : S company Multiplex PCR kit, Supplier I : I company Taq polymerase for Mutiplex PCR (0.5U, added 2mM MgCl₂)Rxn. Condition : 95°C for 10min, followed by 35cycles of 30sec at 95°C, 30sec at 65°C, 60sec at 72°C M; 25/100 bp Mixed DNA Ladder (cat. No. D-1020)

Figure 4. Comparison of amplification quality using Lapchip® between AccuPower® Multiplex PCR PreMix and other supplier’s master mixture.

a) Virtual Gel Image. Gel image illustrates data reproducibility of the LabChip 90 system.
b) Overlay of expression level using Bioneer’s multiplex PCR PreMix and other company’s multiplex PCR kit. The electropherogram displays the data between 10 PCR products yield using 10-plex primer sets to illustrate the Amplification efficiency.
c) The graph shows the total concentration of PCR products between AccuPower® Multiplex PCR PreMix and other supplier’s master mixture.


Figure 5. Comparison of amplification quality between AccuPower® Multiplex PCR PreMix and other supplier’s Multiplex PCR kit.

20-plex primers were added into AccuPower® Multiplex PCR PreMix and other supplier’s Multiplex PCR kit. A series of Human genomic DNA diluents were tested. (100ng ~ 1ng). All data were obtained using Mygene 96 machine (Applied Bioneer co.)
Supplier Q : Q company Multiplex PCR kit, Supplier S : S company Multiplex PCR kit, Supplier I : I company Taq polymerase for Mutiplex PCR (0.5U, added 2mM MgCl₂)
Rxn. Condition : 95°C for 10min, followed by 35cycles of 30sec at 95°C, 30sec at 57°C, 60sec at 72°C M; 25/100 bp Mixed DNA Ladder (cat. No. D-1020) Trademark: 'AccuPower®' is a registered trademark of Bioneer Corporation.

Manual
• AccuPower® Multiplex PCR PreMix

MSDS
• AccuPower® Multiplex PCR PreMix

Brochure
• Amplification New Product-2011 Brochure

Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001:2008 - certificate

• EN ISO 13485:2003 / AC:2009 - certificate