DNA/RNA Amplification Kits


AccuPower® PreMix series is an innovative PCR master mix product range designed to make your PCR, reverse transcription (cDNA amplification) and RT-PCR experiments easier and more cost effective than ever. With a wide range of applications and unparalleled ease of use and stability, AccuPower® PreMix PCR master mix series will be an important addition to your collection of research tools.

Each tube or plate you order has all the components of a PCR master mix (enzyme, buffer, dNTPs) lyophilized with a patented stabilizer that maintains full activity for over one month at room temperature and up to two years in the freezer. Simply add your primers and template – and never make a dNTP or PCR enzyme stock solution again!

In addition, AccuPower®’s reaction stability is maintained at 95°C for up to 3 times longer than Taq DNA polymerase, making it an ideal enzyme for amplifying G:C rich DNA at temperatures above 50°C.

AccuPower® PreMix PCR master mix is available with or without loading dye. When loading dye is present you can load it directly into your gel following PCR – no glycerol or glucose is required.
 

Features and Benefits

  •  Fast: just add primer and template
  •  Convenient: no more dNTP or enzyme stock solutions required
  •  Flexible: available in tube (0.2 or 0.5 ml) or plate (96 or 384 well) formats
  •  Stable: for up to 2 years in the freezer and one month at room temperature
  •  Customizable: available with primers already added for even greater convenience
  •  Gel loading : available with or without tracking dye for ease of use
     


 

]]> DNA Amplification

  Amplification PCR PreMix Taq PCR PreMix Hotstart PCR PreMix Pyro Hotstart Taq PCR PreMix    Pfu PCR PreMix Multiplex PCR PreMix Gold Multiplex PCR PreMix
PCR Standard PCR √ √ √ √   √ √
Hot-start PCR     √ √   √ √
High-fidelity PCR         √    
Long-range PCR              
GC rich PCR     √ √      
Real-Time PCR              
Multiplex PCR     √ √   √ √


RNA Amplification

  Amplification RT PreMix Cycle Script RT PreMix Rocket Script RT PreMix Rocket Script Cycle RT PreMix RT/PCR PreMix RocketScript RT/PCR PreMix Rocket Plex RT/PCR PreMix
PCR Standard PCR         √ √ √
RT Standard RT √ √ √ √ √ √ √
Cycle RT   √   √   √ √
RT/PCR Standard RT/PCR         √ √ √
Multiplex RT/PCR             √


Real time PCR

  Amplification GreenStar
qPCR PreMix		 2X GreenStar
qPCR master Mix		 DualStar
qPCR PreMix
PCR Standard PCR      
Hot-start PCR      
High-Fidelity PCR      
Long-range PCR      
GC rich PCR      
Real-Time PCR √ √ √
MultiplexPCR      


Specifications of AccuPower PreMix series

Product Product Size Yield Specificity Fidelity Conven-
ience		 GCrich Heat
Stability
Lambda Human
PCR PreMix ≤ 10 kb ≤ 3 kb ***** *** *** ***** *** ***
Taq PCR PreMix ≤ 10 kb ≤ 3 kb ***** *** *** ***** *** ***
Hotstart PCR PreMix ≤ 12 kb ≤ 3 kb **** **** *** ***** **** ****
PyroHotstart Taq PCR PreMix ≤ 12 kb ≤ 3 kb **** **** *** ***** **** ****
Pfu PCR PreMix ≤ 20 kb ≤ 10 kb *** *** **** ***** *** ***
ProFi Taq PCR PreMix ≤ 30 kb ≤ 21 kb *** **** ***** ***** *** ****
Multiplex PCR PreMix - 1~20 frag. **** **** *** ***** *** ****
Gold Multiplex PCR PreMix - 1~20 frag. **** **** *** ***** *** ****
RT PreMix - < 9 kb *** - - ***** *** ***
CycleScript RT PreMix - < 9 kb **** - - ***** *** ***
RocKetScript™ RT PreMix - < 10kb **** - - ***** **** ****
RocKetScript™ Cycle RT PreMix - < 10kb **** - - ***** **** ****
RT/PCR PreMix - < 6 kb **** - - ***** *** ***
RocKetScript™ RT/PCR PreMix - < 6 kb **** - - ***** *** ****
RocketPlex RT/PCR PreMix - < 6 kb **** **** - ***** *** ****
GreenStar™ qPCR PreMix - - *** **** - ***** **** ****
2X GreenStar™ qPCR master Mix - - *** **** - ***** **** ****
DualStar™ qPCR PreMix - - *** ***** - ***** **** ****

 

 















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