AccuPrep® His-tagged Protein Purification Kit


Fast micro scale purification of 6xHis-tagged protein

Bioneer's Ni-NTA magnetic silica resins are silica beads, with an average diameter of 1.29 um and a range of 1~5 um diameter, have Ni-NTA (nickel-nitrilotriacetic acid) covalently bound to their surface.
These Ni-NTA magnetic silica beads exhibit outstanding affinity to His-tagged proteins and feature our SSMB (Spherical-Shaped Magnetic Bead) technology that provides increased surface area (due to the small size of the beads), without the issue of impurity carryover common with magnetic particles that have a rough-surface.
Ni-NTA magnetic agarose beads are supplied as a 10% (v/v) suspension with an average binding capacity of 500 ug protein per ml of suspension for 6xHis-tagged protein.


Features and Benefits

  • Flexible: The protocol may be carried out with a magnet or via centrifugation.
  • Excellent performance: Highly pure proteins are obtained through our exclusive SSMB (Spherical-Shape Magnetic Beads).
  • High purity and High yield: Average binding capacity of 500 μg protein per ml of suspension for 6xHis-tagged protein. Purity that is > 90%.
  • Reproducibility: The only hands-on step is the addition of protein extract, maximizing reproducibility.
  • Fast process: The entire purification is complete in about 30 minutes!
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Application


Figure 1. Comparison of Protein Tyrosine Phosphatase(25 kD) purified using AccuPrep® His-tagged Protein Purification Kit and two market leading competitors' products.
AccuPrep® His-tagged Protein Purification Kit outperformed two competitors' similar products in terms of yield and purity.
Lane M: AccuLadder™ Protein Size Marker (Broad)
Lane 1: Crude extract
Lane 2, 5: Bioneer
Lane 3, 6: Company Q
Lane 4, 7: Company E
Washing: Samples taken after the washing step
Elution: eluted protein samples


Figure 2. Various sized proteins purified using AccuPrep® His-tagged Protein Purification Kit.

Proteins as small as 10 kD and as large as 120 kD can be purified with the AccuPrep® His-tagged Protein Purification Kit.
Lane M: AccuLadder™ Protein Size Marker (Broad)
Lane 1: TLA DNA Polymerase
Lane 2: PTPase #54
Lane 3: PTPase pk6


Figure 3. Concentration comparison of TLA DNA polymerase proteins purified using AccuPrep® His-tagged Protein Purification Kit and two market leading competitors' products.

AccuPrep® His-tagged Protein Purification Kit showed up to 5-times higher concentration than that of the competitors.


Procedure





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Manual

AccuPrep® His-tagged Protein Purification Kit


MSDS

AccuPrep® Ni-NTA binding-washing buffer

AccuPrep® Ni-NTA Elution buffer


Brochure

• AccuPrep® His-tagged Protein Purification Kit


Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

ISO 9001:2008 - certificate

EN ISO 13485:2003 / AC:2009 - certificate

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