AccuBead™ Silica Coated Magnetic Bead

Spherical-shaped magnetic beads for superior results

Many research institutes and industries are applying magnetic beads into their applications. They use this material either for cell-based experiments or nucleic acid purification for disease monitoring as well as developing treatments (among other uses). Bioneer's magnetic beads have an average diameter of 1.29 um with a range of 1~5 um diameter and can be coated with
a variety of functional groups. The functional groups allow binding of, for example, DNA, RNA, protein or specific cells.
By using an external magnetic field, the magnetic beads can be separated with their bound products for isolation and purification.

 

Features and Benefits

Bead Size:
The average particle size of silica coated magnetic beads is 1-5 um. AccuBead™ provides the optimal size for attaching
bio-molecules for research, purification and functional studies.

Shape and Uniformity:
AccuBead™ s completely spherical unlike most other magnetic beads. The average particle size distribution is comparatively uniform, and the smooth surface eliminates carryover of impurities common to rough-surfaced beads.

Efficiency:
Because the silica magnetic beads are spherical and uniform, DNA/RNA or protein purification can be performed with
outstanding efficiency and reproducibility.
The magnetic property of the bead allows an external magnetic field to be applied for quick separation.

Reproducibility:
Each batch is produced under strict quality controls. Errors that commonly occur during mass production are eliminated during
the individual packaging process. Bioneer's current batch processing system allows for the production of more accurate and reproducible end-product yield.

Stability:
The product is stable for 3 years under appropriate storage conditions. (Ultra-pure water or buffer – room temperature; Streptavidin Magnetic Beads & Biotin Magnetic Beads – refrigerated).

Applications

Magnetic DNA/RNA Purification
Magnetic Protein Separation
Magnetic Antibody Separation
Magnetic Cell Isolation

Patents

Patent pending: KR Patent 10-2008-0013545
Patent registered: KR Patent 10-1053023
Patent pending: WO 2009/102171 A2
Patent pending: US 2011/0054162 A1

FE-SEM Images of Magnetic Beads

Figure 1. FE-SEM image of magnetic particles for nucleic acid purification.
The uniformity of the magnetic beads can be seen in the micrograph.

Figure 2. FE-SEM magnification image of magnetic beads.
SEM images show complete silica coating of the iron oxide nano particles in spherical form.

Size Distribution and Zeta Potential Diagram of Magnetic Beads

Figure 3. Size distribution diagram and zeta potential of silica coated magnetic beads.
The average size of magnetic beads is approximately 1.56 µm and zeta potential is -39.8 mV due to the hydroxyl group on the surface of the silica.

Magnetic Beads are a highly efficient tool for DNA isolation

DNA/RNA affinity to magnetic beads (uniform-dispersed super-paramagnetic particles) is used for separation.
Magnetic beads are mixed with naked DNA/RNA in high-salt-containing buffer.
DNA/RNA is bound to magnetic beads via a salt bridge.
By magnetic separation, highly enriched DNA/RNA preparations are obtained in a matter of minutes.
Magnetic beads are highly suitable for automation since it requires no centrifugation or vacuum filtration procedures.

First, subject the biological samples to a lysis reaction and place the magnetic beads in the mixture to combine the free DNA together with magnetic beads. Fix magnetic beads with an external magnetic field to wash the solution and then elute
the DNA or RNA from the magnetic beads. Using magnetic beads to for purification is much faster than other methods such as gravity columns or centrifugation.

Figure 4. DNA separation workflow using silica coated magnetic beads.

Electrophoresis Results of purified DNA

Figure 5. Electrophoresis image of silica coated magnetic bead-purified genomic DNA (amplified).

Comparison of viral RNA Prep Kit Quality

Figure 6. Comparison of viral RNA Prep Kit Quality using WHO HIV-1 working standard (PWS-1,2,3) serums.
Superior results are shown when using the AccuPrep™ Viral RNA Kit made with AccuBead™ Silica Coated Magnetic Beads.

Brochure

• AccuBead™ PDF Brochure
 

MSDS

• AccuBead™ Silica Coated Magnetic Beads MSDS

Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001:2008 - certificate

• EN ISO 13485:2003/AC:2009 - certificate

Cat.No.	Product Description	Price	Add to Cart
TS-1000-1	Magnet 15x1.5 and Screw Tube 1.5ml, 1 set	Inquire
TS-1010-1	Silica Coated Magnetic Bead, size 1-5µm, 0.5g/25ml	Inquire
TS-1010-2	Silica Coated Magnetic Bead, size 1~5µm, 1g/50ml	Inquire
TS-1010-3	Silica Coated Magnetic Bead, size 1~5µm, 10g/500ml	Inquire
TS-1011-1	NH2 Magnetic Beads, size 1~5µm, 0.5g/25ml	Inquire