AccuPrep® Viral RNA Extraction Kit

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For various clinical sample applications; Spin column based Viral RNA Extraction Kit – AccuPrep® Viral RNA Extraction Kit

AccuPrep® Viral RNA Extraction Kit is designed for the rapid and convenient extraction of viral RNA from cell-free samples as serum, plasma, CSF, urine, etc. This kit can be used for extracting RNA from a variety of RNA viruses, such as HIV, HAV, HCV, enteroviruses.
NB: not all viruses can be utilized as a source. Also, as it is difficult to extract only RNA from samples containing both DNA and RNA, a cell-free sample is required in order to prevent DNA contamination.

Features and Benefits

Highly purified and high purity viral RNA can be extracted from various clinical samples (whole blood, CSF, urine, swab…).

Silica based RNA binding column with high RNA binding efficiency.

Poly (A) (provided) can protect RNA degradation during the extraction steps and enhance the binding efficiency.

Contents

Components	K-3033 (100 reactions)
Binding buffer (VB)	60 ml X 1 ea
Wahsing buffer 1 (W1)	40 ml X 1 ea
Washing buffer 2 (W2)	20 ml X 1 ea
Elution buffer (EL)	10 ml X 1 ea
Poly (A), lyophilized	2 mg X 1 ea
RNA binding column tube	100 ea X 1 pack
2.0ml tubes for filtration	100 ea X 1 pack
1.5ml tubes for elution	1 ea

Applications

cDNA synthesis, RT-PCR, Quantative real-time RT-PCR, poly A+ RNA selection, Northern blot analysis, pathogen detection

Procedure

Experimental Data

Fig. 1 The RT-PCR results of HCV-specific RNA obtained from HCV-positive serum by using AccuPrep® Viral RNA Extraction Kit.
Lane M; Molecular weight marker, 100bp ladder (D-1030, Bioneer), Lane1, 2; 216bp HCV product