ExiPrep™ 16 Plus

Description

ExiPrep™ 16 Plus is a laboratory automation system able to extract DNA or RNA from various biological samples including tissue, cell culture, whole blood, serum, plasma, swab, Bacteria, yeast, etc. It also features the ability to purify His-tag recombinant proteins. ExiPrep™ 16 Plus can handle up to 16 samples at once and includes optimized extraction protocols for many sample types so you get the unsurpassed results from your nucleic acid or protein purification. The ExiPrep™ 16 Plus has advanced features such as an intuitive touch screen a UV sterilization cycle and a unique contamination shield, each designed to provide an extra level of confidence in your results.

Features and Benefits

Preinstalled Optimized Protocols for Maximum Results

ExiPrep™ 16 Plus contains over 900 optimized protocols for nucleic acid extraction and protein purification. Each protocol is fine-tuned to sample and target type to give anybody reproducible results every experiment.
The instrument software can also be upgraded through the network connection port so you can stay up-to-date with the best performing protocols.

Easy-to-use Touchscreen interface

The responsive 3.5 inch touchscreen enables simple instrument operation. From selecting sample-optimized protocols to turning the UV sterilization lamp on and off, the touchscreen is designed for optimal workflow. During instrument operation, the touchscreen will give real-time feedback on the progress of the extraction, maximizing user convenience.
 

Automatic UV Sterilization Lamp

The ExiPrep™ 16 Plus has a powerful UV sterilization lamp that enables the user to sterilize the instrument chamber before and/or after every nucleic acid extraction or protein purification run. This prevents possible inter-assay cross-contamination that may occur on a busy work day.

Contamination Shield

ExiPrep™ 16 Plus comes with a contamination shield designed to protect the assay from cross-contamination during instrument operation. Any time the pipette tips are moving, the contamination shield will slide under the tips, therefore eliminating the possibility of intra-assay cross-contamination which is critical when working with multiple samples.

Combined Magnetic/Heating Block

To increase nucleic acid extraction and protein purification efficiency, the ExiPrep™ 16 Plus has an integrated combined magnetic/heating block. The combination of bead magnetization and sample heating reduces experiment time and increases elution efficiency, resulting in increased sample yield. (Patent pending)Process: Read clockwise.

 

Experimental Procedure

Applications

Specifications

Comparisons

Experimental Data

1. Genomic DNA from cultured mammalian cell (HeLa)
  Sample volume: 1X10⁶ cells
  Protocol number: 109
  Total prep time: about 1hr 30min.
  Average yield: 4-8ug

Procedure



Agarose gel analysis

Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 1X10⁶ cells of cultured HeLa cell and Lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH₂O as a negative control in DNA extraction.
Note all samples have very similar yields. Purity was also tested and was consistently between 1.8 and 2.0 (not shown).

2. Genomic DNA from Bacteria (E.coli)
  Sample volume: 1X10⁹ cells
  Protocol number: 109
  Total prep time: about 1hr 40min.
  Average yield: 8-12ug
 

Procedure

Agarose gel analysis

Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 1X10⁹ cells of E.coli cells and Lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH₂O as a negative control in DNA extraction.
Note all samples have very similar yields. Purity was also tested and was consistently between 1.9 and 2.0 (not shown).

3. Genomic DNA from Tissue (Bovine skeletal muscle)
  Sample volume: ~25mg
  Protocol number: 102
  Total prep time: about 1hr 30min.
  Average yield: up to 20ug

Procedure

Agarose gel analysis

Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 25mg of bovine tissues and Lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH₂O as a negative control in DNA extraction.
Note all samples have very similar yields. Purity was also tested and was consistently between 1.9 and 2.0 (not shown).

4. Genomic DNA from Plant Tissue (Spinach leaf)
  Sample volume: ~100mg
  Protocol number: 104
  Total prep time: about 1 hr
  Average yield: up to 5ug

Procedure

Agarose gel analysis

Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 100mg of spinach leaves and Lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH₂O as a negative control in DNA extraction.
Note all samples have very similar yields. Purity was also tested and was consistently between 1.9 and 2.0 (not shown).

5. RNA extraction from cultured mammalian cells (HeLa).
  Sample volume: 1.0 X 10⁶ cells
  Protocol number: 202
  Average yield: up to 10 ug
  Average RQS: 9.5

Procedure

Electrophoresis result with LabChip GX

Results of capillary electrophoresis of total RNA extracted from HeLa cell(1X10⁶ cells) using LabChip GX(Caliper life science, USA). Average RQS(RNA Quality Score) were at least 9.5t shown).

Real-time RT-PCR results of total RNA extracted from cultured cell

Real-time RT-PCR results using an AccuPower® RocketScrpt™ RT PreMix(K-2101, Bioneer) and AccuPower® Dualstar™ qPCR PreMix(K-6110, Bioneer) and run on Exicycler™ 96 Real-Time PCR System (A-2060, Bioneer) from total RNA extracted from HeLa cell(1X10⁶ cells)

Manual

• ExiPrep™ 16 Plus

Brochure

• ExiPrep™ 16 Plus

Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001:2008 - certificate

• EN ISO 13485:2003 / AC:2009 - certificate

• CE - certificate