Custom siRNA's according to your specs, or let us design them using our software at no extra cost
Bioneer's Custom siRNA synthesis service offers exceptional quality siRNAs to knock down your target genes. Custom siRNAs can be synthesized according to sequence information you provide, or you can take advantage of our complimentary siRNA design service. Up to 30-mer siRNA including a choice of 32 different 3' overhangs can be ordered with a variety of modification options for expanded specificity. siRNA is provided purified, annealed, lyophilized and ready-to-use. For even greater convenience, check out our AccuTarget™ Genome-wide Pre-designed siRNA - siRNAs pre-designed for human (17,842 genes), mouse (17,118 genes) and rat (9,392 genes) synthesized and ready-to-ship.
All custom siRNAs are synthesized in our state-of-the-art clean room facility and then purified free of charge utilizing Bioneer's BioRP purification system. For higher purity, HPLC purification is available at an additional charge. Each siRNA is quality controlled by MALDI-TOF mass spectrometry to guarantee highest quality and analyzed by PAGE to confirm its duplex structure.
Features and Benefits
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Guaranteed performance: Two of three custom siRNA will give 80% siRNA knockdown*
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Guaranteed Quality: Manufactured in a state-of-the-art clean room and QC’ed by MALDI-TOF and PAGE
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Custom siRNA design service available: Turbo si-Designer software design is available free of charge**
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Competitive pricing: Overhang and annealing service provided free of charge, great value for your research dollar
* 80% siRNA knockdown guarantee policy is not applied to custom siRNA design for noncoding RNA by Turbo si-Designer.
** Complimentary siRNA design service is available only when a customer orders the corresponding siRNA(s) from Bioneer.
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Discover the Bioneer advantage
Gene silencing by RNA interference (RNAi) technology using small interfering RNA (siRNA) is a powerful tool for studying functional genomics, drug target identification and validation, pathway elucidation, and therapeutic development. The Bioneer advantage is in the algorithm we use for siRNA design. Our Turbo si-Designer software identifies highly effective siRNA target sites with remarkably high siRNA knockdown rates. Critical parameters including base composition, thermodynamic instability and base preference are all considered in the design algorithm. siRNAs spanning SNP sites are removed and finally, non-specific siRNAs are eliminated following homology searching by BLAST and Smith-Waterman algorithms. The result is an siRNA with extraordinary siRNA knockdown efficiency and minimal off-target effects. The performance of Turbo si-Designer has been extensively evaluated by testing the siRNA knockdown efficiency of thousands of siRNAs targeting STE Kinases, TK Kinases, genes involved in the NF-kappaB and Caspase Pathways using Real-time PCR analysis. The results of the evaluation indicated the Bioneer's design algorithm was highly effective in selecting effective siRNAs; 80% of the tested siRNAs showed > 70% knockdown and 38% elicited knockdown of > 90%.
Figure 1. siRNA knockdown efficiency of AccuTarget™ Genome-wide Predesigned siRNAs. HeLa cells were transfected with siRNAs at 100 nM.
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Brochure
• siRNA Products and services 2010
Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
• ISO 9001:2008 - certificate
• EN ISO 13485:2003 / AC:2009 - certificate
• EC Directive 98/79/EC - certificate
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|
Cat. No. |
Guaranteed Yield |
Purification |
Price |
|
S-1017-1 |
10 nmole |
BioRP |
$180.00 |
|
S-1017-2 |
20 nmole |
$230.00 |
|
S-1017-3 |
50 nmole |
$350.00 |
|
S-1017-4 |
100 nmole |
$410.00 |
|
S-1018-1 |
10 nmole |
HPLC |
$300.00 |
|
S-1018-2 |
20 nmole |
$410.00 |
|
S-1018-3 |
50 nmole |
$500.00 |
|
S-1018-4 |
100 nmole |
$540.00 |
- siRNA provided lyophilized and annealed.
- Annealing buffer is provided when single-stranded siRNA requested.
|
Modification |
10 nmole |
20 nmole |
50 nmole |
100 nmole |
|
5' Fluorescein |
$80.00 |
$90.00 |
$120.00 |
$170.00 |
|
5' Phosphorylation |
$50.00 |
$60.00 |
$75.00 |
$120.00 |
|
5' Biotin |
$80.00 |
$90.00 |
$120.00 |
$170.00 |
|
5' Amine |
$50.00 |
$60.00 |
$75.00 |
$120.00 |
|
5' TAMRA |
$180.00 |
$220.00 |
$260.00 |
$340.00 |
|
5'-Cy3 |
$230.00 |
$290.00 |
$340.00 |
$440.00 |
|
5'-Cy5 |
$230.00 |
$290.00 |
$340.00 |
$440.00 |
|
5' Thiol |
$100.00 |
$120.00 |
$150.00 |
$210.00 |
|
5' PEG 2000 |
$450.00 |
$580.00 |
$760.00 |
$980.00 |
|
3' Fluorescein |
$80.00 |
$90.00 |
$120.00 |
$170.00 |
|
3' Phosphorylation |
$60.00 |
$70.00 |
$85.00 |
$130.00 |
|
3' Biotin |
$130.00 |
$150.00 |
$180.00 |
$250.00 |
|
3' Amine |
$60.00 |
$70.00 |
$85.00 |
$130.00 |
|
3' TAMRA |
$180.00 |
$220.00 |
$260.00 |
$340.00 |
|
3' Thiol |
$100.00 |
$120.00 |
$150.00 |
$210.00 |
|
3' DABCYL |
$180.00 |
$220.00 |
$260.00 |
$340.00 |
|
3' Cholesterol |
$130.00 |
$150.00 |
$180.00 |
$250.00 |
|
3' PEG 2000 |
$450.00 |
$580.00 |
$760.00 |
$980.00 |
|
Internal 2'-F(A) |
$40.00 |
$50.00 |
$60.00 |
$60.00 |
|
Internal 2'-F(C) |
$40.00 |
$50.00 |
$60.00 |
$60.00 |
|
Internal 2'-F(G) |
$40.00 |
$50.00 |
$60.00 |
$60.00 |
|
Internal 2'-F(U) |
$40.00 |
$50.00 |
$60.00 |
$60.00 |
|
Internal Phosphorothioate |
N/A |
$5.50 |
$9.00 |
$12.00 |
|
Internal 2'-OMe |
$12.00 |
$17.00 |
$25.00 |
$35.00 |
|
Internal Inosine |
$25.00 |
$30.00 |
$40.00 |
$50.00 |
|
Internal Deoxy-abase |
$70.00 |
$90.00 |
$110.00 |
$150.00 |
|
Chimeric DNA Bases |
$0.50 |
$0.80 |
$1.20 |
$2.00 |
- Certain items may not be available in all countries.
- Order Custom siRNA now.
CUSTOM SIRNA SYNTHESIS
Let Bioneer design your siRNA with Turbo si-Designer, or use the form below to create your own. Up to 30-mer siRNA including a choice of up to different modifications can be ordered at each scale (10, 20, 50 and 100 nmol guaranteed yields) at no extra charge. Each siRNA synthesized by Bioneer is purified to near HPLC purity utilizing the company's proprietary BioRP purification system. For higher purity, HPLC is offered at an additional charge. The siRNA is supplied lyophilized and ready-to-use for your convenience.
Each siRNA is quality controlled by MALDI-TOF mass spectrometry to guarantee highest quality and analyzed by PAGE to confirm its duplex structure. Bioneer offers 7 different 5' modifications: Fluorescein, Phosphorylation, Biotin, Amine, TAMRA, Thiol and PEG 2000. Bioneer also offers 9 different 3' modifications: Fluorescein, Phosphorylation, Biotin, Amine, TAMRA, Thiol, DABCYL, Cholesterol and PEG 2000. 6 internal modifications are also available: DNA, 2'-O-Me, 2'-FU, 2'-FC, Inosine and Deoxy-base. All Bioneer's siRNA products are manufactured in a state-of-the-art clean room.
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