Positive and Negative control siRNA's for your experiments. > 90% siRNA knockdown for positive controls
The AccuTarget™ Positive Control siRNAs are designed to induce high siRNA knockdown of their target genes. siRNA targeting an endogenous gene (GAPDH) and a reporter system (GFP and Luciferase) are available. AccuTarget™ Negative Control siRNAs do not target any known genes in human, mouse and rat. The negative control siRNA can be fluorescently labeled for easier monitor of transfection efficiency. AccuTarget™ Control siRNA Sets consisting of a positive and negative control siRNAs are also available for user convenience.
Features and Benefits
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Excellent performance: Positive control siRNA knockdown rates > 90%
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Monitoring of transfection rate: Convenient fluorescently labeled negative control sets
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Competitive pricing: Great value for your research dollar
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Positive and Negative Control siRNA
A) Northern Blotting B) qRT-PCR
Figure 1. Effects of Human GAPDH Positive Control siRNA. HeLa cells were transfected separately with AccuTarget™ Human GAPDH Positive Control and Negative Control siRNA using Lipofectamine 2000 (Invitrogen) at a final concentration of 100 nM. Total cellular RNA was isolated from transfected cells 24 hours after transfection and subjected to Northern blot and Real-Time PCR analysis. As can be seen from Fig. 1-B, about 3% GAPDH mRNA remained.
GFP Control siRNA
Figure 2. HeLa cells in a 24-well plate were cotransfected with 200 ng of CMV-GFP plasmid and 10 nM of GFP siRNA using lipofectamine 2000 transfection reagent. Next day, the expression of GFP was observed by using a Nikon Eclipse TS100 epifluorescence microscope. In contrast to bright green fluorescence of GFP protein in NC-siRNA-transfected cells, no fluorescence was detected from GFP-siRNA-transfected cells, indicating efficient knockdown of GFP by using our positive control GFP-siRNA.
GFP Control siRNA
Figure 3. HeLa cells in a 6-well plate were cotransfected with 400 ng of CMV-luc plasmid and 10 nM of luciferase siRNA using lipofectamine 2000 transfection reagent. Next day, cells were harvested and assayed for luciferase activity. As shown in Fig. 3, cotransfection with our positive control luciferase siRNA led to efficient knockdown of luciferase activity (85% - 95% knockdown compared to luciferase activity of NC-siRNA-transfected cells).
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Brochure
• siRNA Products and services 2010
Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
• ISO 9001:2008 - certificate
• EN ISO 13485:2003 / AC:2009 - certificate
• EC Directive 98/79/EC - certificate
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