• Standard Oligos


For routine applications, Bioneer offers standard custom Oligos without modification in tube format from  25 nmole scale upwards at very competitive pricing. 
Bio-RP (near-HPLC) purification is included at no additional charge and these oligos can be ordered online or via e-mail from this website with simplicity.

Features and Benefits

  • Free Bio-RP purification: Near HPLC purity at a standard oligo price
  • Quick turnaround time: < 3.5 business days from order to bench in most cases
  • Competitive pricing: Great value for your research dollar
  • Quality that you can rely on

Why is Bio-RP purification better than desalting?

Most oligos sold are merely deprotected/desalted. The process of deprotection/desalting only partially purifies the oligo and leaves behind many impurities* and failure products (truncated oligos that will not amplify and that may interfere with certain applications). These can lead to artificially inflated OD readings that increase with oligo length.
Bio-RP purification removes these contaminants and failure products, resulting in an oligo that demonstrates near HPLC purity. The advantage to you is that our product contains only quality oligos minus impurities that may inhibit some PCR reactions and that potentially skew qPCR reactions.
We deliver the same amount of active oligo as our competitors; they just have more “other” artificially inflating their OD.
To demonstrate this, plates of Oligos of various length were tested for concentration in 2 steps:
1) following a deprotection/desalting step only, and 2) following Bio-RP purification. (Fig. 1)

The results of these tests show that the amount of failed sequences and impurities* increases with length (as expected), but the failure product is not removed by deprotection/desalting alone. This results in inflated OD readings for oligos depending on oligo length.
(Table 1)


Figure 1.
Plates of Oligos of various length were tested for concentration following desalting and then by Bio-RP cartridge purification.
Note inflated OD readings when deprotection/desalting alone is used.

Oligo Length (# bases) 18 - 24 24 - 29 30 - 34 35 - 41
OD Inflation 38% 45% 60% 77%

Table 1. Average OD inflation for oligos of various length when protection/desalting alone is employed v. Bio-RP purified product.
Note for longer oligos; up to 77% of product does not consist of functional oligo.

*Impurities in desalted oligos may include: Acetonitrile, Pyridine, Iodine,
Ethylthiotetrazole, Dichloromethane, Acetic acid, Acrylonitrile, Benzamide and Isobutyramide.



 

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Standard Oligo Pricing

Synthesis Scale (μmole) Price/Base
($)
Base Limitation(mer) Purification Price
($)
Guaranteed Yield (OD)
[ based on 20 bases ]
Approximate Working Days to Ship
BioRP PAGE HPLC BioRP PAGE HPLC BioRP PAGE HPLC
0.025 0.29 15-60 Free - - 2 1 1.5 2 3 3
0.05 0.47 10-75 Free 40.00 40.00 4 2 2.5 2 3 3
0.2 0.92 5-110 Free 59.00 59.00 8 6 7 2 3 3
1 1.75 5-130 Free 100.00 100.00 30 18 25 2 3 3
10 14.00 5-50 Free 280.00 280.00 300 150 200 2 3 3
15 22.00 5-50 Free 420.00 420.00 Inquire Inquire Inquire 3 4 4
* The synthesis scales refer to the initial starting point for a synthesis. The starting synthesis scale and the length of the oligo will influence the final yield.


For orders larger than 96 oligos (High-Throughput) pricing, click here.

For Modification oligos pricing, click here.

For Dual-labeled Probes pricing, click here.

 

Additional Services - Pricing

Post-Handling Tube Price ($) Plate Price ($)
Concentration normalized-Standard 0.30 0.15 (per well)
Concentration normalized-Custom 0.40 0.20 (per well)
Bar-code Label No Charge No Charge
Dried No Charge No Charge
Aliquoting to daughter Plates 0.40 0.20 (per well)
Mixing primers 0.40 0.20 (per well)
Individual Tube plate-Labeled - 0.40 (per well)
Custom plate - 10.00
Double strand (Annealing service) 30.00/Pair -
MALDI-TOF (QC) No Charge No Charge
MALDI-TOF (SNP or Genotyping) - 2.00 (per well)
Shipping Charge Please see 'Ordering / Shipping' Please see 'Ordering / Shipping'
Hand mix service (MIX base Code) 100.00 (1~250base) 50.00 (1~250base)
Remainder in plate service -  95.00 (96 well Plate),
150.00 (384 well Plate)
Circular ssDNA service 300.00/oligo 225.00 / oligo

Custom Analytical Service

Items Tube Price ($) Plate Price ($) large scale
I-E HPLC 50.00 2.50 (per well) 50.00
RP HPLC 50.00 2.50 (per well) 50.00
Capillary electrophoresis 50.00 2.50 (per well) 50.00
PAGE analysis 15.00 1.00 (per well) 15.00
MALDI-TOF analysis, Spectrocheck - 2.00 (per well) -
MALDI-TOF analysis, other mass system Free Free Free

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Manual

Treatment for thiol modified oligonucleotides

Anneal complementary pairs of oligonucleotides


MSDS

Oligos


Brochure

Oligonucleotides 2010 Brochure


FAQ

Oligo FAQ


Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

ISO 9001:2008 - certificate

EN ISO 13485:2003 / AC:2009 - certificate

EC Directive 98/79/EC - certificate

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