AccuZolâ„¢ Total RNA Extraction Solution


RNA Extraction – AccuZol™ Total RNA Extraction Solution



AccuZol™ is a ready-to-use reagent for the isolation of total RNA from various sample materials.
A monophasic solution of phenol and guanidine-salt inhibits RNase activity, and so the reagent maintains integrity of the RNA during sample homogenization or lysis.
AccuZol™ allows you to extract high-yield total RNA with small quantities of starting materials.

Features and Benefits

Simplicity
AccuZol™ minimize the steps handle hazardous reagents so that RNase/DNase contamination keeps to a minimum.

Experiment Time
The procedure for total RNA isolation can be completed in 1 hour.

Sample Source
Tissues and cultured cells originated from human, animals, plant, and bacteria, or blood samples.

Easy Phase Separation
Green colored reagent is useful to distinguish between organic phase and aqueous phase.

Application
cDNA synthesis, RT-PCR, Real-time RT-PCR, Northern/dot blot analysis, RNase protection assays, Molecular cloning


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Specifications
  Samples Starting volume Elution volume Average yield (ug)
Animal Tissue Liver
Kidney
Spleen
Lung
Brain
10 mg 50ul 50 - 70
25 - 40
40 - 60
15 - 25
8 - 12
Cultured Cell HeLa
A549
PC3
1 x 106 cells 50ul 15 - 30
15 - 25
15 - 25
Bacterial Cell E.coli 1 x 107 cells 50ul 10 - 15
Plant Tissue Bean leaf 100 mg 50ul 30 - 50
Blood Human whole blood 250 ul 50ul 2 - 3

 

Applications

cDNA synthesis, Northern blotting, RT-PCR, real time RT-PCR

 

Procedure




Experimental Data


Fig. 1 Electrophoresis Pattern of total RNA extracted from animal tissues by AccuZol

Total RNA was isolated from 10-20mg of rat tissues and 2 ug of total RNA was loaded per lane on a 1% denaturing agarose gel.


Fig. 2 Electrophoresis Pattern of total RNA extracted from human cell lines by AccuZol

Total RNA was isolated from 1x106 cultured cells and 1 ug of total RNA was loaded per lane on a 1% denaturing agarose gel.



Fig. 3 Electrophoresis Pattern of total RNA extracted from plant by AccuZol

Total RNA was isolated from 100mg of bean leaves and 1 ug of total RNA was loaded per lane on a 1% denaturing agarose gel.


Fig. 4 RT & PCR analysis of total RNA extracted from rat tissues by AccuZol

This analysis was performed with RT/PCR Premix (Bioneer, K-2055) and rat GAPDH primer. The electrophoresis pattern shows clear bands PCR products


Fig. 5 RT & PCR analysis of total RNA extracted from human cell lines by AccuZol

This analysis was performed with RT/PCR Premix (Bioneer, K-2055) and human GAPDH primer. The electrophoresis pattern shows clear bands PCR products.
 

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Manual

AccuZol™ Total RNA Extraction Solution


Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

ISO 9001:2008 - certificate


 

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