Plasmid DNA Mini Prep – AccuPrep® Plasmid Mini Extraction Kit
The AccuPrep® Plasmid Extraction Kit is designed for the rapid extraction of high-purity plasmid DNA from bacterial cultures such as E.coli. As a column-type tube is utilized in the purification process, extraction is carried out in three simple steps of binding/ washing/ elution, and use of dangerous organic solvents is avoided for the safe and convenient extraction of high-purity plasmid DNA.
Features and Benefits
Silica based DNA binding column with high DNA binding capacity
Endonuclease A denaturation buffer for the endA+ strains (Denaturation Buffer, Buffer D).
Based on the modified alkaline lysis method
50 well tube rack for the storage of the extracted plasmid DNA
Contents
|
Components |
K-3030 (200 reactions) |
K-3030-1 (50 reactions) |
|
Buffer ① (Resuspension buffer) |
60 ml X 1 ea |
15 ml X 1 ea |
|
Buffer ② (Lysis buffer) |
60 ml X 1 ea |
15 ml X 1 ea |
|
Buffer ③ (Neutralization buffer) |
80 ml X 1 ea |
20 ml X 1 ea |
|
Buffer ④ (Washing buffer) |
16 ml X 2 ea |
4 ml X 2 ea |
|
Buffer ⑤ (Elution buffer) |
24 ml X 1 ea |
6 ml X 1 ea |
|
Buffer D (Denaturation buffer) |
75 ml X 1 ea |
18 ml X 1 ea |
|
RNase A, lyophilized |
6 mg X 1 ea |
1.5 mg X 1 ea |
|
DNA binding column tube |
50 ea X 4 box |
50 ea X 1 box |
|
Manual |
1 ea |
1 ea |
|
One Page Protocol |
1 ea |
1 ea |
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Specifications
|
Starting culture volume |
1 ml ~ 10 ml |
|
Column binding capacity |
> 20 ug |
|
Elution volume |
50 – 100 ul |
|
Expected DNA yield |
Upto 20 ug |
|
Preparation time |
< 10 min |
Applications
Sub-cloning, Sequencing, Transformation, Trasnfection, In-vitro transcription/ translation
Procedure
Experimental Data
Fig. 1 Improved Yield
Plasmid DNA purified with the AccuPrep® Nano-Plus Plasmid Mini Extraction Kit AccuPrep® Nano-Plus Mini Kit provides high yields of plasmid DNA.
Lane B; AccuPrep® Plasmid Mini Extraction Kit, Lane 2, 3; competitors
Fig. 2 Restriction enzyme digestion test
200ng of extracted plasmid DNA(pBluescript SK(+)) was digested with XbaI. Each enzymes completely recognize its restriction enzyme site and digest.
Lane M; Molecular weight marker(λDNA/HindIII+EcoRI, Cat. No. D-1070, Bioneer)
Lane 1; AccuPrep® Plasmid Mini Extraction Kit Lane 2, 3; competitors
200ng of extracted plasmid DNA(pBluescript SK(+)) was digested with various restriction enzymes. Each enzymes completely recognize its restriction enzyme site and digest.
Lane M; Molecular weight marker(λDNA/HindIII+EcoRI, Cat. No., D-1070, Bioneer)
K; Kpn I, X; Xho I, SI; Sal I, H; Hind III, E; EcoR I, P; Pst I, Sm; Sma I, B; Bam HI, S; Spe I, Xb; Xba I, N; Not I, Sc;Sac I
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Manual
• AccuPrep® Plasmid Mini Extraction Kit
MSDS
• AccuPrep
Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
• ISO 9001:2008 - certificate
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