Full length cDNA synthesis and PCR followed by AccuPower® RocketScript™ RT-PCR PreMix
AccuPower® RocketScript™ RT-PCR PreMix contains Bioneer’s exclusive M-MLV based thermostable reverse transcriptase (RTase) and Top DNA polymerase, dNTPs and reaction buffer components all in a single, easy to use, one-step RT-PCR product. By using RocketScript™ RTase, full-length cDNA are efficiently synthesized from complex secondary structure RNA species. RocketScript™ also has outstanding extension properties, allowing for highly-sensitive and high-yielding cDNA synthesis of low copy targets. The convenient lyophilized PreMix also contains tracking dye (blue and yellow) and also a density-increasing agent, allowing the user to directly place the reaction mixture in an agarose gel and perform electrophoresis.
Features and Benefits
Thermostable Activity:
Original M-MLV RTase has maximum activity at relatively low temperatures (42 °C), causing several problems in reverse transcription of complex secondary structure RNA molecules. To remedy this issue, Bioneer has developed a RTase active at high temperatures (above 50°C). RocketScript™ has thermostable activity (42~70°C), allowing efficient cDNA synthesis from complex secondary structure RNA and give the user freedom to optimize the reverse transcription reaction based on temperature.
Ease-of-Use:
- The product contains our thermostable RTase RocketScript™ , RNase inhibitors and all other components necessary for
a successful RT reaction in a single tube. Just add RNA, primers and PCR-grade water to perform the RT reaction.
-Components necessary for agarose gel electrophoresis are also contained within the product including tracking dye and
a density-increasing reagent for added convenience.
-T vector cloning is possible without any additional reactions.
Reproducibility:
Each product batch is produced under strict quality control processes for accurate and reproducible end product manufacturing.
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Specifications
5' to 3' exonuclease: No
3' to 5' exonuclease: No
3’ – A Overhang: Yes
Fragment Size: 6 kb
Application
- First-strand synthesis of cDNA from RNA molecules (RT)
- RT-PCR
- Library construction
- Gene-expression analysis
Experimental Data
Figure 1. Performance comparison between AccuPower® RocketScript™ RT-PCR PreMix and competitor RT-PCR kits
Complex secondary structure RNA species amplification by RocketScript™ is outstanding compared to the leading competitor’s reverse transcriptase. RT reactions were performed according to each manufacturer’s recommendations.
Lanes 1-4 are 10 ng, 1 ng, 0.1 ng and 0.01 ng of total RNA from HeLa cells, respectively
Figure 2. AccuPower® RocketScript™ RT-PCR PreMix shows enhanced performance compared to competitors
One-Step RT-PCR reactions were performed with 100ng total RNA from HeLa cells using regents and conditions specified in each manufacturer’s protocol
Lane 1: 2.5kb Lane 2: 3kb Lane 3: 4.5kb Lane 4: 5.2kb
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Manual
• AccuPower® RocketScript™ RT-PCR PreMix
MSDS
• AccuPower® RocketScript™ RT-PCR PreMix
Brochure
• AccuPower® RocketScript™ RT-PCR PreMix - 2011 Brochure
Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
• ISO 9001:2008 - certificate
• EN ISO 13485:2003 / AC:2009 - certificate
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