For routine applications, Bioneer offers standard custom Oligos without modification in tube format from 25 nmole scale upwards at very competitive pricing.
Bio-RP (near-HPLC) purification is included at no additional charge and these oligos can be ordered online or via e-mail from this website with simplicity.
Features and Benefits
-
Free Bio-RP purification: Near HPLC purity at a standard oligo price
-
Quick turnaround time: < 3.5 business days from order to bench in most cases
-
Competitive pricing: Great value for your research dollar
-
Quality that you can rely on
Why is Bio-RP purification better than desalting?
Most oligos sold are merely deprotected/desalted. The process of deprotection/desalting only partially purifies the oligo and leaves behind many impurities* and failure products (truncated oligos that will not amplify and that may interfere with certain applications). These can lead to artificially inflated OD readings that increase with oligo length.
Bio-RP purification removes these contaminants and failure products, resulting in an oligo that demonstrates near HPLC purity. The advantage to you is that our product contains only quality oligos minus impurities that may inhibit some PCR reactions and that potentially skew qPCR reactions.
We deliver the same amount of active oligo as our competitors; they just have more “other” artificially inflating their OD.
To demonstrate this, plates of Oligos of various length were tested for concentration in 2 steps:
1) following a deprotection/desalting step only, and 2) following Bio-RP purification. (Fig. 1)
The results of these tests show that the amount of failed sequences and impurities* increases with length (as expected), but the failure product is not removed by deprotection/desalting alone. This results in inflated OD readings for oligos depending on oligo length.
(Table 1)
Figure 1. Plates of Oligos of various length were tested for concentration following desalting and then by Bio-RP cartridge purification.
Note inflated OD readings when deprotection/desalting alone is used.
|
Oligo Length (# bases) |
18 - 24 |
24 - 29 |
30 - 34 |
35 - 41 |
|
OD Inflation |
38% |
45% |
60% |
77% |
Table 1. Average OD inflation for oligos of various length when protection/desalting alone is employed v. Bio-RP purified product.
Note for longer oligos; up to 77% of product does not consist of functional oligo.
*Impurities in desalted oligos may include: Acetonitrile, Pyridine, Iodine,
Ethylthiotetrazole, Dichloromethane, Acetic acid, Acrylonitrile, Benzamide and Isobutyramide.
]]>
Standard Oligo Pricing
|
Synthesis Scale (μmole) |
Price/Base
($) |
Base Limitation(mer) |
Purification Price
($) |
Guaranteed Yield (OD)
[ based on 20 bases ] |
Approximate Working Days to Ship |
|
BioRP |
PAGE |
HPLC |
BioRP |
PAGE |
HPLC |
BioRP |
PAGE |
HPLC |
|
0.025 |
0.29 |
15-60 |
Free |
- |
- |
2 |
1 |
1.5 |
2 |
3 |
3 |
|
0.05 |
0.47 |
10-75 |
Free |
40.00 |
40.00 |
4 |
2 |
2.5 |
2 |
3 |
3 |
|
0.2 |
0.92 |
5-110 |
Free |
59.00 |
59.00 |
8 |
6 |
7 |
2 |
3 |
3 |
|
1 |
1.75 |
5-130 |
Free |
100.00 |
100.00 |
30 |
18 |
25 |
2 |
3 |
3 |
|
10 |
14.00 |
5-50 |
Free |
280.00 |
280.00 |
300 |
150 |
200 |
2 |
3 |
3 |
|
15 |
22.00 |
5-50 |
Free |
420.00 |
420.00 |
Inquire |
Inquire |
Inquire |
3 |
4 |
4 |
* The synthesis scales refer to the initial starting point for a synthesis. The starting synthesis scale and the length of the oligo will influence the final yield.
For orders larger than 96 oligos (High-Throughput) pricing, click here.
For Modification oligos pricing, click here.
For Dual-labeled Probes pricing, click here.
Additional Services - Pricing
|
Post-Handling |
Tube Price ($) |
Plate Price ($) |
|
Concentration normalized-Standard |
0.30 |
0.15 (per well) |
|
Concentration normalized-Custom |
0.40 |
0.20 (per well) |
|
Bar-code Label |
No Charge |
No Charge |
|
Dried |
No Charge |
No Charge |
|
Aliquoting to daughter Plates |
0.40 |
0.20 (per well) |
|
Mixing primers |
0.40 |
0.20 (per well) |
|
Individual Tube plate-Labeled |
- |
0.40 (per well) |
|
Custom plate |
- |
10.00 |
|
Double strand (Annealing service) |
30.00/Pair |
- |
|
MALDI-TOF (QC) |
No Charge |
No Charge |
|
MALDI-TOF (SNP or Genotyping) |
- |
2.00 (per well) |
|
Shipping Charge |
Please see 'Ordering / Shipping' |
Please see 'Ordering / Shipping' |
|
Hand mix service (MIX base Code) |
100.00 (1~250base) |
50.00 (1~250base) |
|
Remainder in plate service |
- |
95.00 (96 well Plate),
150.00 (384 well Plate) |
|
Circular ssDNA service |
300.00/oligo |
225.00 / oligo |
Custom Analytical Service
|
Items |
Tube Price ($) |
Plate Price ($) |
large scale |
|
I-E HPLC |
50.00 |
2.50 (per well) |
50.00 |
|
RP HPLC |
50.00 |
2.50 (per well) |
50.00 |
|
Capillary electrophoresis |
50.00 |
2.50 (per well) |
50.00 |
|
PAGE analysis |
15.00 |
1.00 (per well) |
15.00 |
|
MALDI-TOF analysis, Spectrocheck |
- |
2.00 (per well) |
- |
|
MALDI-TOF analysis, other mass system |
Free |
Free |
Free |
]]>
Manual
• Treatment for thiol modified oligonucleotides
• Anneal complementary pairs of oligonucleotides
MSDS
• Oligos
Brochure
• Oligonucleotides 2010 Brochure
FAQ
• Oligo FAQ
Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
• ISO 9001:2008 - certificate
• EN ISO 13485:2003 / AC:2009 - certificate
• EC Directive 98/79/EC - certificate
]]>