T7 RNA Polymerase

T7 RNA Polymerase is isolated from E.coli cells containing the ligase gene cloned from T7 bacteriophage.

T7 RNA Polymerase is a DNA dependent RNA polymerase with a highly specificity for the initiation of transcription at T7 RNA polymerase promoters. It is widely used for the rapid synthesis in vitro of specific RNAs.

Features and Benefits

Source: T7 RNA Polymerase is isolated from E.coli cells containing the ligase gene cloned from T7 bacteriophage.

]]> Applications

- Synthesis of RNA transcripts for northern hybridization and southern hybridization probes.
- RNA generation for studies of RNA structure, procession and catalysis

Supplied with Enzyme

- 10 x Reaction Buffer (1 ml): 400 mM Tris-HCl (pH 8.0), 60 mM MgCl₂, 20 mM Spermidine
- 100 mM DTT (0.5 ml)
- DEPC-DW (1ml)

Storage Condition

200 mM Na-phosphate (pH 7.7), 10 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.02 % Triton x -100, 0.08% Tween-20, 50% Glycerol, Store at -20°C

Concentration

20.1 units/㎕

Unit Definition

One unit of enzyme catalyzed incorporation of 1 nmole of [3H]ATPs into acid insoluble form in 60 min at 37°C

Quality Assurance

Nuclease Contamination Assay	No altered was detected after incubation of 1 ug of substrate DNA with 500 units of T7 RNA polymerase for 18 hrs in 37°C
Protease Contamination Assay	No altered pattern was detected after in cubation of 2,000 units of T7 RNA polymerase for 18 hrs in 37°C

]]> Manual

• T7 RNA Polymerase

MSDS

• T7 RNA Polymerase

• T7 RNA Polymerase-reaction buffer

• DEPC DW

Brochure

• Enzymes 2010 Brochure

Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001:2008 - certificate

• EN ISO 13485:2003 / AC:2009 - certificate

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