ProFi Taq DNA polymerase

Features and Benefits

• Long PCR: ProFi Taq is especially effective in amplifying large genomic DNA fragments around 20 kb and amplifying Lambda DNA up to 30kb.

• Flexible: ProFi Taq provides accurate long-range amplification of standard and amplification of low-copy target, and is highly suitable for all PCR applications.

• Reproducibility: Each batch is produced under strict quality controls. Errors that commonly occur during mass production are eliminated during the individual packaging process. Bioneer’s current batch processing system allows for the production of more accurate and reproducible end-product yield.

5' to 3' exonuclease activity: Yes
3' to 5' exonuclease activity: No
3’ – A Overhang: Yes
PCR product size: ~ 30 kb

Application

- Primer extension
- long-range amplification from genomic DNA
- High amplification efficiency
- Excellent performance on difficult templates
- Amplification of low-copy targets
- High yield and high sensitivity PCR

Experimental data


Figure 1. Comparison of PCR amplification efficiency between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase.
The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5min, 30 cycles of 95°C for 20 sec, 55°C for 20 sec and 72°C for 30sec. PCR reaction using other suppliers' DNA polymerase were performed according to each supplier's protocol.

Target: human Insulin receptor gene.
Lane M: 100 bp DNA Ladder(Bioneer, Cat. No. D-1030)
Lane 1: 10 ng of human genomic DNA
Lane 2: 1 ng of human genomic DNA
Lane 3: 100 pg of human genomic DNA
Lane 4: 10 pg of human genomic DNA



Figure 2. Comparison of PCR amplification efficiency between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase
cDNA synthesized from 10-fold serial-diluted human total RNA from 10 ng to 10 pg using AccuPower® RocketScript™ Cycle RT PreMix. (Bioneer, Cat. No K-2201) wase used as a template for PCR amplification. The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5min, 33 cycles of 95°C for 20 sec, 55°C for 20 sec and 72°C for 30 sec. PCR reactions using other suppliers' DNA polymerase were performed according to each supplier's protocol.

Target: human GAPDH gene.
Lane M: 100 bp DNA Ladder(Bioneer, Cat. No. D-1030)
Lane 1: 10 ng of human total cDNA
Lane 2: 1 ng of human total cDNA
Lane 3: 100 pg of human total cDNA
Lane 4: 10 pg of human total cDNA



Figure 3. Comparison of PCR amplification of long targets between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase
The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5 min, 30 cycles of 95°C for 20 sec, 65°C for 20 sec and 68°C for 4 min. PCR reactions using other suppliers' DNA polymerase were performed according to each supplier's protocol.

Lane M1: Lambda/Hind III marker (Bioneer, Cat. No. D-1050)
Lane M2: 1 kb DNA Ladder (Bioneer, Cat. No. D-1040)
Lane 1: 2 kb fragment (human tumor protein p53 gene)
Lane 2: 3 kb fragment (human tumor protein p53 gene)
Lane 3: 4.5kb fragment (human DNA cross-link repair 1A gene)
Lane 4: 8 kb fragment (human hemoglobin epsilon 1 gene)



Figure 4. Comparison of PCR amplification of long targets between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase
The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5 min, 32 cycles of 95°C for 20 sec and 68°C for 15 min. PCR reactions using other suppliers' DNA polymerase were performed according to each supplier's protocol.
Human genomic DNA was used as a template for PCR amplification.

Lane M1: Lambda/Hind III marker (Bioneer, Cat. No. D-1050)
Lane M2: 1 kb DNA Ladder (Bioneer, Cat. No. D-1040)
Lane 1: 11 kb fragment
Lane 2: 13.5 kb fragment
Lane 3: 17.6 kb fragment
Lane 4: 21.4 kb fragment



Figure 5. Comparison of PCR amplification of long targets between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase
The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5 min, 32 cycles of 95°C for 20 sec, 65°C for40 sec, and 68°C for 20 min. PCR reactions using other suppliers' DNA polymerase were performed according to each supplier's protocol. Lambda DNA was used as a template for PCR amplification.

Lane M1: Lambda/Hind III marker (Bioneer, Cat. No. D-1050)
Lane M2: 1 kb DNA Ladder (Bioneer, Cat. No. D-1040)
Lane 1: 15 kb fragment
Lane 2: 20 kb fragment
Lane 3: 25 kb fragment
Lane 4: 30 kb fragment

• ProFi Taq polymerase

MSDS

• ProFi Taq polymerase

Brochure

• ProFi Taq polymerase Brochure

Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001:2008 - certificate

• EN ISO 13485:2003 / AC:2009 - certificate