Hotstart Taq DNA Polymerase


An Antibody-based HotStart Taq DNA Polymerase for increased specificity and robust sensitivity




HotStart Taq DNA Polymerase is designed to increase specificity and sensitivity in PCR. The HotStart Taq DNA polymerase is inhibited at temperatures lower than 70°C, but is fully activated after the first denaturation step. This prevents the formation of mis-primed products and primer-dimers during the reaction setup process, resulting in improved PCR specificity.

Features and Benefits

  • Maximized specificity: Virtually eliminates non-specific amplification. Ideal for multiplex PCR with 2-6 amplicons.
  • Improved sensitivity: Ecellent for PCR using low copy number targets
  • TA cloning compatible: PCR products amplified with Hotstart Taq DNA polymerase have 3' A overhang and can be used for TA cloning.
  • Versatility: HotStart Taq DNA Polymerase is ideal for a wide range of PCR applications.

 

]]>Enzyme Properties

Concentration: 5 U/ul
5' to 3' exonuclease activity: Yes
3' to 5' exonuclease activity: No
3' – A Overhang: Yes
Nuclease contamination: Certified DNase and RNase free
Extension rate: 3–10 kb/minute depending on template complexity
Fragment Size: Up to 10 kb

 

Application

- Multiplex PCR - Hotstart PCR - Routine PCR - SYBR-Green-based qPCR - Dual-labeled probe based qPCR - Real-Time quantification of DNA and cDNA targets using SYBR Green dye - Primer extension - TA cloning - Gene sequencing - SNP

 

Kit Contents

Cat. No. HotStart Taq DNA Polymerase 10 x Rxn Buffer with MgCl2 Dilution Buffer dNTP Mixture
E-2017 250 units 0.5 ml 0.5 ml 0.5 ml
E-2017-1 1,000 units 4 x 0.5 ml 4 x 0.5 ml 4 x 0.5 ml
E-2017-2 500 units 2 x 0.5 ml 2 x 0.5 ml 2 x 0.5 ml
E-2017-3 250 units 0.5 ml 0.5 ml -
E-2017-4 1,000 units 4 x 0.5 ml 4 x 0.5 ml -

- 10 x Reaction Buffer: Tris-HCl, KCl, 15 mM MgCl2, pH 9.0
- Dilution Buffer: 20 mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 100 mM KCl, Stabilizers, 50 % Glycerol, pH 8.0
- dNTPs mixture: 10 mM, each dNTP 2.5 mM

 

Storage Conditions

HotStart Taq DNA Polymerase, including buffers and reagents, should be stored immediately upon receipt at –20°C.
If stored in the recommended temperature, this product will be stable until the expiration date printed out on the label.

 

Unit Definition

One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 72°C.

 

Experimental data

Figure 1. Singleplex and multiplex PCR were carried out to amplify fragments of different sizes from human genomic DNA p53 gene.

Lane M: 100 bp DNA Ladder of Bioneer ( Cat.No D-1030)
Lane 1: 139bp fragment Lane 5: 1,082bp fragment
Lane 2: 211bp fragment Lane 6: 1,296bp fragment
Lane 3: 447bp fragment Lane 7: 1,561bp fragment
Lane 4: 618bp fragment Lane 8: Multiplex PCR of 139, 447, and 618 bp fragments


Figure 2. Performance comparison between Bioneer HotStart Taq DNA polymerase and competitors' HotStart DNA polymerase.

Lane M: 100 bp DNA Ladder of Bioneer ( Cat.No D-1030)
Lane 1: 139bp fragment Lane 5: 1,082bp fragment
Lane 2: 211bp fragment Lane 6: 1,296bp fragment
Lane 3: 447bp fragment Lane 7: 1,561bp fragment
Lane 4: 618bp fragment Lane 8: Multiplex PCR of 139, 447, and 618 bp fragments

Bioneer: Bioneer HotStart Taq DNA polymerase
A: Competitor A's HotStart DNA polymerase
B: Competitor B's HotStart DNA polymerase

]]> Manual

• Hotstart Taq DNA Polymerase


MSDS

• Hotstart Taq DNA Polymerase


Brochure

• Hotstart Taq DNA Polymerase Brochure


Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001:2008 - certificate

• EN ISO 13485:2003 / AC:2009 - certificate

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