AccuPrep® Nano-Plus Plasmid Midi Extraction Kit

A novel Plasmid DNA Extraction Kit utilizing unique technology from Bioneer – AccuPrep® Nano-Plus Plasmid Midi Extraction Kit : 30 min


The AccuPrep® Nano-Plus Plasmid Midi Extraction Kit extracts highly-purified plasmid DNA from cultured bacterial cells in approximately 40 minutes. The principle utilizes a modified alkaline lysis protocol that is enhanced by a novel, patented Nano-Technology from Bioneer.

Features and Benefits

Principle is based on modified alkaline lysis protocol combined with a novel nano- particle technology

Suitable for both high-copy and low copy number plasmid DNA.

Highly purified and high purity plasmid DNA can be extracted from cultured E.coli cells within 40 min.

 

Contents

Specifications

 

Applications

Sub-cloning, Sequencing, Transformation, Trasnfection, In-vitro transcription/ translation

 

Procedure


 

Principle

ExiPrep™ Blood Genomic DNA Kit is a product designed to extract high-purity genomic DNA from whole blood samples using the automated nucleic acid extraction instrument ExiPrep™ 16 Plus, ExiProgen™. The lysis buffer and Proteinase K contained within the product will efficiently lysis cells and hydrolysis proteins within whole blood for efficient extraction of genomic DNA. The binding buffer and silica magnetic particles will bind genomic DNA to the surface of the beads. The reaction containers are then subject to a magnetic field, forcing the genomic DNA-bound silica magnetic particles to remain within the container while the reaction mixture and cellular waste are removed. Three types of washing buffers are introduced sequentially during the next step to remove the remainder of cellular waste products. The washing buffers are then siphoned off, while the genomic DNA-bound silica magnetic particles remain bound by the magnetic field. DNase-free elution buffer is then introduced to the silica magnetic particles to release the bound and now purified genomic DNA.

 

Experimental Data

Fig. 1 Reduced Total Prep. Time
The nano-particles which included in the Buffer ①(Resuspension buffer) can make a complex together with insoluble protein aggregate, cell debris and chromosomal DNA in the solution. And the complex can be separated from the solution with simple centrifuge step(5min.) to obtain a cleared lysate which contains target plasmid DNA.
B; AccuPrep® Nano-Plus Plasmid Midi Extraction Kit, Q, P; Competitors

Fig. 2 Improved Prep. Yield
Plasmid DNA purified with the AccuPrep® Nano-Plus Plasmid Mini Extraction Kit AccuPrep® Nano-Plus Midi Kit provides high yields of plasmid DNA.
B; AccuPrep® Nano-Plus Plasmid Midi Extraction Kit, P, Q; competitors 

Fig. 3 Restriction enzyme digestion test

Electrophoresis results with extracted plasmid DNA after restriction enzyme digestion with various restriction enzymes.
pBS; pBluescript SK(+) with XbaI, pBI121; pBI121 with PstI, Lane M; Molecular Marker (λ-DNA/ HindIII+EcoRI, Cat. No. D-1070, Bioneer), Lane B; AccuPrep® Nano-Plus Plasmid Midi Extraction Kit, Lane Q, P; competitors


Electrophoresis results with extracted plasmid DNA after restriction enzyme digestion with various restriction enzymes.
Left; pBluescript SK(+), Right; pBI121, Lane M; Molecular Marker (Cat. No. D-1040, Bioneer)


 

Manual

• AccuPrep® Nano-Plus Plasmid Extraction Kit (Mini/Midi/Maxi)

MSDS

• AccuPrep

Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001:2008 - certificate

Cat.No.	Product Description	Price	Add to Cart
K-3111	AccuPrep® Nano-Plus Plasmid Mini Extraction Kit, 200 reactions	Inquire
K-3112	AccuPrep® Nano-Plus Plasmid Mini Extraction Kit, 50 reactions	Inquire