A novel Plasmid DNA Extraction Kit utilizing unique technology from Bioneer – AccuPrep® Nano-Plus Plasmid Mini Extraction Kit : 10 min
The
AccuPrep® Nano-Plus Plasmid Mini Extraction Kit extracts highly-purified plasmid DNA from cultured bacterial cells in approximately 10 minutes. The overall principle utilizes a modified alkaline lysis protocol that is enhanced by novel, patented Nano-Technology from Bioneer.
Features and Benefits
Principle is based on modified alkaline lysis protocol combined with a novel nano- particle technology
Suitable for both high-copy and low copy number plasmid DNA.
Highly purified and high purity plasmid DNA can be extracted from cultured E.coli cells within 10 min.
Endonuclease A denaturation buffer for the endA+ strains (Denasturation Buffer, Buffer D).
Silica based DNA binding column with high DNA binding efficiency.
Contents
Components |
K-3111 |
K-3112 |
(200 reactions) |
(50 reactions) |
Buffer ① (Resuspension buffer) |
60 ml X 1 ea |
15 ml X 1 ea |
Buffer ② (Lysis buffer) |
60 ml X 1 ea |
15 ml X 1 ea |
Buffer ③ (Neutralization buffer) |
80 ml X 1 ea |
20 ml X 1 ea |
Buffer ④ (Washing buffer) |
16 ml X 2 ea |
4 ml X 2 ea |
Buffer ⑤ (Elution buffer) |
24 ml X 1 ea |
6 ml X 1 ea |
Buffer D (Denaturation buffer) |
75 ml X 1 ea |
18 ml X 1 ea |
RNase A, lyophilized |
6 mg X 1 ea |
1.5 mg X 1 ea |
DNA binding column tube |
50 ea X 4 box |
50 ea X 1 box |
Manual |
1 ea |
1 ea |
One Page Protocol |
1 ea |
1 ea |
Specifications
Starting culture volume |
1 ml ~ |
Column binding capacity |
> 20 ug |
Elution volume |
50 – 100 ul |
Expected DNA yield |
Upto 20 ug |
Preparation time |
< 10 min |
Applications
PCR, real time PCR, Cloning, SNP analysis, Pharmacogenomics, Genetics
Procedure
Principle
ExiPrep™ Blood Genomic DNA Kit is a product designed to extract high-purity genomic DNA from whole blood samples using the automated nucleic acid extraction instrument ExiPrep™ 16 Plus, ExiProgen™. The lysis buffer and Proteinase K contained within the product will efficiently lysis cells and hydrolysis proteins within whole blood for efficient extraction of genomic DNA. The binding buffer and silica magnetic particles will bind genomic DNA to the surface of the beads. The reaction containers are then subject to a magnetic field, forcing the genomic DNA-bound silica magnetic particles to remain within the container while the reaction mixture and cellular waste are removed. Three types of washing buffers are introduced sequentially during the next step to remove the remainder of cellular waste products. The washing buffers are then siphoned off, while the genomic DNA-bound silica magnetic particles remain bound by the magnetic field. DNase-free elution buffer is then introduced to the silica magnetic particles to release the bound and now purified genomic DNA.
Experimental Data
Fig. 1 Reduced Total Prep. Time
The nano-particles which included in the Buffer ①(Resuspension buffer) can make a complex together with insoluble protein aggregate, cell debris and chromosomal DNA in the solution. And the complex can be separated from the solution with simple centrifuge step(1min.) to obtain a cleared lysate which contains target plasmid DNA.
B; AccuPrep® Nano-Plus Plasmid Mini Extraction Kit, Q, P; Competitors
Fig. 2 Improved Prep. Yield
Plasmid DNA purified with the AccuPrep® Nano-Plus Plasmid Mini Extraction Kit AccuPrep® Nano-Plus Mini Kit provides high yields of plasmid DNA.
B; AccuPrep® Nano-Plus Plasmid Mini Extraction Kit, P, Q; competitors
Fig. 3 Restriction enzyme digestion test
Electrophoresis results with extracted plasmid DNA after restriction enzyme digestion with various restriction enzymes. pBS - pBluescript SK(+) with XbaI, pBI121 -pBI121 with PstI, Lane M - Molecular Marker(λ-DNA/ HindIII+EcoRI, Cat. No. D-1070, Bioneer), Lane B - AccuPrep® Nano-Plus Plasmid Mini Extraction Kit, Lane Q, P - competitors
Electrophoresis results with extracted plasmid DNA after restriction enzyme digestion with various restriction enzymes. Left - pBluescript SK(+), Right pBI121, Lane M - Molecular Marker(Cat. No. D-1040, Bioneer)
Manual
• AccuPrep® Nano-Plus Plasmid Extraction Kit (Mini/Midi/Maxi)
MSDS
• AccuPrep
Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
• ISO 9001:2008 - certificate
Cat.No.
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Product Description
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Price
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Add to Cart
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K-3111
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AccuPrep® Nano-Plus Plasmid Mini Extraction Kit, 200 reactions |
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Inquire
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K-3112
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AccuPrep® Nano-Plus Plasmid Mini Extraction Kit, 50 reactions
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Inquire
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