AccuPrep® PCR Purification Kit

For various enzymatic reaction product, Spin column type fragment DNA Purification Kit – AccuPrep® PCR Purification Kit


AccuPrep® PCR Purification Kit is designed for the purification of up to 10 ug of DNA fragment from PCR and other enzymatic products within 5 minutes. The size range for effective purification is 100 bp ~10 kb, thus common 20 ~ 40 mer oligonucleotides are removed. The recovery yield exceeds 70~90%. Elution volume can be as little as 30 uL when concentrated product is needed.

Features and Benefits

Completely purify the fragment DNA from various enzymatic reaction products within 5 min.

Highly purified and high yield fragment DNA can be purified from various enzymatic reaction products (restriction enzyme digestion, A-tailing, labeling…).

Double strand and single strand DNA can be purified with high recovery.

Usable range is 100bp to 10kb with 70%-90% recovery.

Silica based DNA binding column with high binding efficiency.

 

Contents

Applications

Sub-cloning, Sequencing, Labeling, DNA concentration, etc


Procedure




Experimental Data

Fig. 1 Typical recovery
The figure shows recovery percentage after purification of DNA amplified by PCR.
The 70 % - 90 % of DNA were recovered regardless of DNA size(0.2 - 6.0 kb).
B; AccuPrep® PCR Purification Kit (K-3034, K-3034-1), Q; Competitor


Fig. 2 Recovery analysis of the purified PCR product
Lane M; Molecular weight marker, 100 bp Plus ladder (D-1035, Bioneer),
Lane 100 ~ 50; control DNA(100% ~ 50%),
Lane B; purified PCR product



 

Manual

• AccuPrep® PCR Purification Kit

Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001:2008 - certificate

 

Cat.No.	Product Description	Price	Add to Cart
K-3034	AccuPrep® PCR Purification Kit 200 reactions	Inquire
K-3034-1	AccuPrep® PCR Purification Kit 50 reactions	Inquire