Yeast Genome-Wide Library


S. pombe Genome-wide Deletion Mutant Library

The world's first S. pombe Genome-wide Deletion Mutant Library
This genome-wide S. pombe deletion collection covers more than 98% of genome consisting of 4,914 genes. As each deletion strain has its distinct tags, functional analysis can be performed both in a gene-specific manner and a genome-wide scale. Two different sets of mutant collections are available: h+ haploids and h+/ h+ diploids.


S. cerevisiae VN-Fusion Library

Powerful Tool for Protein-Protein Interaction Analysis
The yeast Saccharomyces cerevisiae(S. cerevisiae) is a recognized simple eukaryote model system which genome can be easily manipulated. The S. cerevisiae VN-Fusion Library was created by Dr. Won-Ki Huh of Seoul National University (Korea). The VN-Fusion Library consists of 5,809 VN-tagged Open Reading Frames (ORFs) covering 93% of the yeast proteome.

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Product     Reference                                            
Name                                                                                                                               
Information              Link
S. pombe Stc1: A Critical Link between RNAi and Chromatin Modification Required for Heterochromatin Integrity Elizabeth H. Bayne, Sharon A. White, Alexander Kagansky, Dominika A. Bijos, Luis Sanchez-Pulido, Kwang-Lae Hoe, Dong-Uk Kim, Han-Oh Park, Chris P. Ponting, Juri Rappsilber and Robin C. Allshire Cell. 2010 Mar 5;140(5):666-77. More
S. pombe Global fitness profiling of fission yeast deletion strains by barcode sequencing Tian Xu Han, Xing-Ya Xu, Mei-Jun Zhang, Xu Peng, and Li-Lin Du Genome Biol. 2010; 11(6): R60. More
S. pombe Improved tools for efficient mapping of fission yeast genes: identification of microtubule nucleation modifier mod22-1 as an allele of chromatin-remodelling factor gene swr1 Andreas Anders, Stephen Watt, Jürg Bähler, and Kenneth E Sawin Yeast 2008 December; 25(12) 913–925 More
S. pombe Functional Genomics of Adhesion, Invasion, and Mycelial Formation in Schizosaccharomyces pombe James Dodgson, Hema Avula, Kwang-Lae Hoe, Dong-Uk Kim, Han-Oh Park, Jacqueline Hayles, and John Armstrong Eukaryot Cell. 2009 August; 8(8): 1298–1306 More
S. pombe Calnexin Is Involved in Apoptosis Induced by Endoplasmic Reticulum Stress in the Fission Yeast Renée Guérin, Geneviève Arseneault, Stéphane Dumont, and Luis A. Rokeach Mol Biol Cell. 2008 October; 19(10): 4404–4420. More
S. pombe Critical Functions of Rpa3/Ssb3 in S-Phase DNA Damage Responses in Fission Yeast Santiago Cavero, Oliver Limbo, and Paul Russell PLoS Genet. 2010 September; 6(9): e1001138. More
S. pombe Genomic Binding Profiling of the Fission Yeast Stress-Activated MAPK Sty1 and the bZIP Transcriptional Activator Atf1 in Response to H2O2 Majid Eshaghi, Jong Hoon Lee, Lei Zhu, Suk Yean Poon, Juntao Li, Kwang-Hyun Cho, Zhaoqing Chu, R. Krishna M. Karuturi, and Jianhua Liu PLoS One. 2010; 5(7): e11620. More
S. pombe The Cell Surface Protein Gene ecm33+ Is a Target of the Two Transcription Factors Atf1 and Mbx1 and Negatively Regulates Pmk1 MAPK Cell Integrity Signaling in Fission Yeast Hirofumi Takada, Aiko Nishida, Mitsuhiro Domae, Ayako Kita, Yuki Yamano, Atsushi Uchida, Shunji Ishiwata, Yue Fang, Xin Zhou, Takashi Masuko, Mitsuhiro Kinoshita, Kazuaki Kakehi, and Reiko Sugiura* Mol Biol Cell. 2010 February 15; 21(4): 674–685. More
S. pombe Expression profiling of S. pombe acetyltransferase mutants identifies redundant pathways of gene regulation Rebecca L Nugent, Anna Johnsson, Brian Fleharty, Madelaine Gogol, Yongtao Xue-Franzén, Chris Seidel, Anthony PH Wright, and Susan L Forsburg BMC Genomics. 2010; 11: 59. More
S. pombe Genome-Wide Screen of Genes Required for Caffeine Tolerance in Fission Yeast Isabel A. Calvo, Natalia Gabrielli, Iván Iglesias-Baena, Sarela García-Santamarina, Kwang-Lae Hoe, Dong Uk Kim, Miriam Sansó, Alice Zuin, Pilar Pérez, José Ayté, and Elena Hidalgo PLoS One. 2009; 4(8): e6619. More
S. pombe Inter-Species Complementation of the Translocon Beta Subunit Requires Only Its Transmembrane Domain Alexandre Leroux and Luis A. Rokeach PLoS ONE. 2008; 3(12): e3880. More
S. pombe A Genome-Wide Screen of Genes Involved in Cadmium Tolerance in Schizosaccharomyces pombe Patrick J. Kennedy, Ajay A. Vashisht, Kwang-Lae Hoe, Dong-Uk Kim, Han-Oh Park, Jacqueline Hayles, and Paul Russell Toxicol Sci. 2008 November; 106(1): 124–139. More
S. pombe A Genetic Engineering Solution to the “Arginine Conversion Problem” in Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)* Claudia C. Bicho, Flavia de Lima Alves, Zhuo A. Chen, Juri Rappsilber, and Kenneth E. Sawin Mol Cell Proteomics. 2010 July; 9(7): 1567–1577. More
S. pombe Plasmid Construction Using Recombination Activity in the Fission Yeast Schizosaccharomyces pombe Ayako Chino, Kenji Watanabe, and Hisao Moriya PLoS One. 2010; 5(3): e9652. More
S. pombe Understanding cytokinesis: lessons from fission yeast Thomas D. Pollard and Jian-Qiu Wu Nat Rev Mol Cell Biol. Author manuscript; available in PMC 2010 August More
S. pombe An acetylated form of histone H2A.Z regulates chromosome architecture in Schizosaccharomyces pombe Hyun-Soo Kim, Vincent Vanoosthuyse, Jeffrey Fillingham, Assen Roguev, Stephen Watt, Thomas Kislinger, Alex Treyer, Laura Rocco Carpenter, Christopher S. Bennett, Andrew Emili, Jack F. Greenblatt, Kevin G. Hardwick, Nevan J. Krogan, Jürg Bähler, and Michael-Christopher Keogh* Nat Struct Mol Biol. Author manuscript; available in PMC 2010 June 1. More
S. pombe Functional Differentiation of tbf1 Orthologues in Fission and Budding Yeasts Moira M. Cockell, Libera Lo Presti, Lorenzo Cerutti, Elena Cano Del Rosario, Philippe M. Hauser, and Viesturs Simanis Eukaryot Cell. 2009 February; 8(2): 207–216. More
S. pombe Functional Characterization of Pneumocystis carinii brl1 by Transspecies Complementation Analysis Libera Lo Presti, Moira Cockell, Lorenzo Cerutti, Viesturs Simanis, and Philippe M. Hauser Eukaryot Cell. 2007 December; 6(12): 2448–2452. More
S. pombe The JmjC domain protein Epe1 prevents unregulated assembly and disassembly of heterochromatin Sarah C Trewick, Elsa Minc, Richard Antonelli, Takeshi Urano, and Robin C Allshire EMBO J. 2007 November 14; 26(22): 4670–4682. More
S. pombe Specific functions for the fission yeast Sirtuins Hst2 and Hst4 in gene regulation and retrotransposon silencing Mickaël Durand-Dubief, Indranil Sinha, Fredrik Fagerström-Billai, Carolina Bonilla, Anthony Wright, Michael Grunstein, and Karl Ekwall EMBO J. 2007 May 16; 26(10): 2477–2488. More
S. pombe Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes Scott J. Dixon, Yaroslav Fedyshyn, Judice L. Y. Koh, T. S. Keshava Prasad, Charly Chahwan, Gordon Chua, Kiana Toufighi, Anastasija Baryshnikova, Jacqueline Hayles, Kwang-Lae Hoe, Dong-Uk Kim Han-Oh Park, Chad L. Myers, Akhilesh Pandey, Daniel Durocher, Brenda J. Andrews, and Charles Boonea Proc Natl Acad Sci U S A. 2008 October 28; 105(43): 16653–16658. More
S. pombe Screening a genome wide S. pombe deletion library identifies novel genes and pathways involved in the DNA damage response Gaurang P. Deshpande, Jacqueline Hayles, Kwang-Lae Hoe, Dong-Uk Kim, Han-Oh Park, and Edgar Hartsuiker DNA Repair (Amst). 2009 May 1; 8(5): 672–679. More
S. pombe S. pombe genome deletion project An update Mario Spirek, Zsigmond Benko, Martina Carnecka, Cornelia Rumpf, Lubos Cipak, Monika Batova, Ivana Marova, Miyoung Nam, Dong-Uk Kim, Han-Oh Park, Jacqueline Hayles, Kwang-Lae Hoe, Paul Nurse, and Juraj Gregan Cell Cycle. 2010 June 15; 9(12): 2399–2402. More
S. pombe Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe Kim DU, Hayles J, Kim D, wood V. Park HO, Won M, Yoo Hs, Duhig T, Nam M, Palmer G, Han S, Jeffery L, BaekST, Lee H, Shim YS, Lee M, Kim L, Heo KS, Noh EJ, LeeAR, Jang YJ, Chung KS, Choi SJ, Park JY, Park Y, Kim HM, Park SK, Park Hj, Kang EJ, Kim HB, Kang HS, Park HM, Kim K, Song K, Song KB, Nurse P, Heo KL Nature Biotechnology. 2012 Jun;28(6): 617-23 More
S. pombe Systematic Screen of Schizosaccharomyces pombe Deletion Collection Uncovers Parallel Evolution of the Phosphate Signal Transduction Pathway in Yeasts Theresa C. Henry, Juliette E. Power, Christine L. Kerwin, Aishat Mohammed, Jonathan S. Weissman, Dale M. Cameron, and Dennis D. Wykoff Eukaryot Cell. 2011 February; 10(2): 198–206. More
S. pombe Cross-species chemogenomic profiling reveals evolutionarily conserved drug mode of action Laura Kapitzky, Pedro Beltrao, Theresa J Berens, Nadine Gassner, Chunshui Zhou, Arthur Wüster, Julie Wu, M Madan Babu, Stephen J Elledge, David Toczyski, R Scott Lokey, and Nevan J Krogan Mol Syst Biol. 2010; 6: 451. More
S. pombe Identification and characterization of the mitochondrial RNA polymerase and transcription factor in the fission yeast Schizosaccharomyces pombe Hengyi Jiang, Wenxia Sun, Zhe Wang, Jing Zhang, Dongrong Chen, and Alastair I. H. Murchie Nucleic Acids Res. 2011 July; 39(12): 5119–5130. More
S. pombe Ubiquitin-proteasome genes as targets for modulation of cisplatin sensitivity in fission yeast Laura Gatti, Kwang L Hoe, Jacqueline Hayles, Sabina C Righetti, Nives Carenini, Laura Dal Bo, Dong U Kim, Han O Park, and Paola Perego BMC Genomics. 2011; 12: 44. More
S. pombe The Essential Functions of NEDD8 Are Mediated via Distinct Surface Regions, and Not by Polyneddylation in Schizosaccharomyces pombe David Girdwood, Dimitris P. Xirodimas, and Colin Gordon PLoS One. 2011; 6(5): e20089. More
S. pombe Reorganization of the Growth Pattern of Schizosaccharomyces pombe in Invasive Filament Formation James Dodgson, William Brown, Carlos A. Rosa, and John Armstrong Eukaryot Cell. 2010 November; 9(11): 1788–1797. More
S. pombe The telomeric transcriptome of Schizosaccharomyces pombe Amadou Bah, Harry Wischnewski, Vadim Shchepachev, and Claus M. Azzalin Nucleic Acids Res. 2012 April; 40(7): 2995–3005. More
S. pombe Break-induced ATR and Ddb1–Cul4Cdt2 ubiquitin ligase-dependent nucleotide synthesis promotes homologous recombination repair in fission yeast Jennifer Moss, Helen Tinline-Purvis, Carol A. Walker, Lisa K. Folkes, Michael R. Stratford, Jacqueline Hayles, Kwang-Lae Hoe, Dong-Uk Kim, Han-Oh Park, Stephen E. Kearsey, Oliver Fleck, Christian Holmberg, Olaf Nielsen, and Timothy C. Humphrey Genes Dev. 2010 December 1; 24(23): 2705–2716. More
S. pombe Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex Emerson V. Stewart, Christine C. Nwosu, Zongtian Tong, Assen Roguev, Timothy D. Cummins, Dong-Uk Kim, Jacqueline Hayles, Han-Oh Park, Kwang-Lae Hoe, David W. Powell, Nevan J. Krogan, and Peter J. Espenshade Mol Cell. 2011 April 22; 42(2): 160–171. More
S. pombe Identification of Genes Affecting the Toxicity of Anti-Cancer Drug Bortezomib by Genome-Wide Screening in S. pombe Kojiro Takeda, Ayaka Mori, and Mitsuhiro Yanagida PLoS One. 2011; 6(7): e22021. More
S. pombe A piggyBac transposon-based mutagenesis system for the fission yeast Schizosaccharomyces pombe Jun Li, Jia-Min Zhang, Xin Li, Fang Suo, Mei-Jun Zhang, Wenru Hou, Jinghua Han, and Li-Lin Du Nucleic Acids Res. 2011 March; 39(6): e40. More
S. pombe The SET Domain Protein, Set3p, Promotes the Reliable Execution of Cytokinesis in Schizosaccharomyces pombe Stefan Rentas, Reza Saberianfar, Charnpal Grewal, Rachelle Kanippayoor, Mithilesh Mishra, Dannel McCollum, and Jim Karagiannis PLoS One. 2012; 7(2): e31224. More
S. pombe Nsk1 ensures accurate chromosome segregation by promoting association of kinetochores to spindle poles during anaphase B Graham J. Buttrick, John C. Meadows, Theresa C. Lancaster, Vincent Vanoosthuyse, Lindsey A. Shepperd, Kwang-Lae Hoe, Dong-Uk Kim, Han-Oh Park, Kevin G. Hardwick, and Jonathan B. A. Millar Mol Biol Cell. 2011 December 1; 22(23): 4486–4502. More
S. pombe A homeodomain transcription factor regulates the DNA replication checkpoint in yeast Frances S Purtill, Simon K Whitehall, Emma S Williams, Christopher J McInerny, Andrew D Sharrocks, and Brian A Morgan Cell Cycle. 2011 February 15; 10(4): 664–670. More
S. pombe Glucosidase II and N-glycan mannose content regulate the half-lives of monoglucosylated species in vivo Ivan D. Stigliano, Solana G. Alculumbre, Carlos A. Labriola, Armando J. Parodi, and Cecilia D'Alessio Mol Biol Cell. 2011 June 1; 22(11): 1810–1823. More
S. pombe Global Gene Expression Analysis of Fission Yeast Mutants Impaired in Ser-2 Phosphorylation of the RNA Pol II Carboxy Terminal Domain Reza Saberianfar, Stephen Cunningham-Dunlop, and Jim Karagiannis PLoS One. 2011; 6(9): e24694. More
S. pombe Genome-Wide Screening for Genes Associated with FK506 Sensitivity in Fission Yeast Yan Ma, Weijuan Jiang, Qingbin Liu, Sayomi Ryuko, and Takayoshi Kuno PLoS One. 2011; 6(8): e23422. More
S. pombe Conservation and Rewiring of Functional Modules Revealed by an Epistasis Map in Fission Yeast Assen Roguev, Sourav Bandyopadhyay, Martin Zofall, Ke Zhang, Tamas Fischer, Sean R. Collins, Hongjing Qu, Michael Shales, Han-Oh Park, Jacqueline Hayles, Kwang-Lae Hoe, Dong-Uk Kim, Trey Ideker, Shiv I. Grewal, Jonathan S. Weissman, and Nevan J. Krogan Science. 2008 October 17; 322(5900): 405–410 More
S. pombe Chromatin-Associated Proteins Revealed by SILAC-Proteomic Analysis Exhibit a High Likelihood of Requirement for Growth Fitness under DNA Damage Stress Han Wang, Pornpimol Tipthara, Lei Zhu, Suk Yean Poon, Kai Tang, and Jianhua Liu Int J Proteomics. 2012; 2012: 630409. More
S. pombe Cross-Species Functionome Analysis Identifies Proteins Associated with DNA Repair, Translation and Aerobic Respiration as Conserved Modulators of UV-Toxicity John P. Rooney, Ashish Patil, Fraulin Joseph, Lauren Endres, Ulrike Begley, Maria R. Zappala, Richard P. Cunningham, and Thomas J. Begley IGenomics. 2011 March; 97(3): 133–147. More
S. pombe Translational Control of Cell Division by Elongator Fanelie Bauer, Akihisa Matsuyama, Julie Candiracci, Marc Dieu, Judith Scheliga, Dieter A. Wolf, Minoru Yoshida, and Damien Hermand Cell Rep. 2012 May 10; 1(5): 424–433. More
S. pombe The CCR4-NOT Complex Is Implicated in the Viability of Aneuploid Yeasts Yoshie Tange, Atsushi Kurabayashi, Bunshiro Goto, Kwang-Lae Hoe, Dong-Uk Kim, Han-Oh Park, Jacqueline Hayles, Yuji Chikashige, Chihiro Tsutumi, Yasushi Hiraoka, Fumiaki Yamao, Paul Nurse, and Osami Niwa PLoS Genet. 2012 June; 8(6): e1002776. More
S. pombe DNA repair factor APLF is a histone chaperone Pawan Vinod Mehrotra, Dragana Ahel, Daniel P. Ryan, Ria Weston, Nicola Wiechens, Rolf Kraehenbuehl, Tom Owen-Hughes, and Ivan Ah Mol Cell. 2011 January 7; 41(1): 46–55. More
S. pombe Studies on the Roles of Clathrin-Mediated Membrane Trafficking and Zinc Transporter Cis4 in the Transport of GPI-Anchored Proteins in Fission Yeast Wurentuya Jaiseng, Yue Fang, Yan Ma, Reiko Sugiura, and Takayoshi Kuno PLoS One. 2012; 7(7): e41946. More
S. pombe Critical Functions of Rpa3/Ssb3 in S-Phase DNA Damage Responses in Fission Yeast Santiago Cavero, Oliver Limbo, and Paul Russell PLoS Genet. 2010 September; 6(9): e1001138. More
S. pombe Response of Schizosaccharomyces pombe to Zinc Deficiency Samantha J. Dainty, Ciara A. Kennedy, Stephen Watt, Jürg Bähler, and Simon K. Whitehall Eukaryot Cell. 2008 March; 7(3): 454–464. More
S. pombe Functional Characterization of Pneumocystis carinii brl1 by Transspecies Complementation Analysis Libera Lo Presti, Moira Cockell, Lorenzo Cerutti, Viesturs Simanis, and Philippe M. Hauser Eukaryot Cell. 2007 December; 6(12): 2448–2452. More
S. pombe An acetylated form of histone H2A.Z regulates chromosome architecture in Schizosaccharomyces pombe Hyun-Soo Kim, Vincent Vanoosthuyse, Jeffrey Fillingham, Assen Roguev, Stephen Watt, Thomas Kislinger, Alex Treyer, Laura Rocco Carpenter, Christopher S. Bennett, Andrew Emili, Jack F. Greenblatt,3,9 Kevin G. Hardwick,2 Nevan J. Krogan,4,5 Jürg Bähler,6 and Michael-Christopher Keogh Nat Struct Mol Biol. 2009 December; 16(12): 1286–1293. More
S. pombe The Cell Surface Protein Gene ecm33+ Is a Target of the Two Transcription Factors Atf1 and Mbx1 and Negatively Regulates Pmk1 MAPK Cell Integrity Signaling in Fission Yeast Hirofumi Takada, Aiko Nishida, Mitsuhiro Domae, Ayako Kita, Yuki Yamano, Atsushi Uchida, Shunji Ishiwata, Yue Fang, Xin Zhou, Takashi Masuko, Mitsuhiro Kinoshita, Kazuaki Kakehi, and Reiko Sugiura Mol Biol Cell. 2010 February 15; 21(4): 674–685. More
S. pombe Dissection of the essential steps for condensin accumulation at kinetochores and rDNAs during fission yeast mitosis Norihiko Nakazawa, Takahiro Nakamura, Aya Kokubu, Masahiro Ebe, Koji Nagao, and Mitsuhiro Yanagida J Cell Biol. 2008 March 24; 180(6): 1115–1131. More
S. pombe Improved tools for efficient mapping of fission yeast genes: identification of microtubule nucleation modifier mod22-1 as an allele of chromatin- remodelling factor gene swr1 Andreas Anders, Stephen Watt, Jürg Bähler, and Kenneth E Sawin Yeast. 2008 December; 25(12): 913–925. More
S. pombe Elimination of a specific histone H3K14 acetyltransferase complex bypasses the RNAi pathway to regulate pericentric heterochromatin functions Bharat D. Reddy, Yu Wang, Lifang Niu, Emily C. Higuchi, Samuel B. Marguerat, Jürg Bähler, Gerald R. Smith, and Songtao Jia Genes Dev. 2011 February 1; 25(3): 214–219. More
S. pombe High-throughput knockout screen in fission yeast Juraj Gregan, Peter K Rabitsch, Cornelia Rumpf, Maria Novatchkova, Alexander Schleiffer, and Kim Nasmyth Nat Protoc. 2006; 1(5): 2457–2464. More
S. pombe The next frontier of systems biology: higher-order and interspecies interactions Michael A Fischbach, Nevan J Krogan Genome Biol. 2010; 11(5): 208. More
S. pombe Specific functions for the fission yeast Sirtuins Hst2 and Hst4 in gene regulation and retrotransposon silencing Mickaël Durand-Dubief, Indranil Sinha, Fredrik Fagerström-Billai, Carolina Bonilla, Anthony Wright, Michael Grunstein, and Karl Ekwall EMBO J. 2007 May 16; 26(10): 2477–2488. More
S. pombe The JmjC domain protein Epe1 prevents unregulated assembly and disassembly of heterochromatin Sarah C Trewick, Elsa Minc, Richard Antonelli, Takeshi Urano, and Robin C Allshire EMBO J. 2007 November 14; 26(22): 4670–4682. More
S. pombe Genomewide identification of pheromone-targeted transcription in fission yeast Yongtao Xue-Franzén, Søren Kjærulff, Christian Holmberg, Anthony Wright, and Olaf Nielsen BMC Genomics. 2006; 7: 303. More
S. pombe Quantitative cell array screening to identify regulators of gene expression Pinay Kainth and Brenda Andrews Brief Funct Genomics. 2010 January; 9(1): 13–23. More
S. pombe The Natural Anticancer Agent Plumbagin Induces Potent Cytotoxicity in MCF-7 Human Breast Cancer Cells by Inhibiting a PI-5 Kinase for ROS Generation Ju-Hee Lee, Ji-Hyun Yeon, Hanna Kim, Whijae Roh, Jeiwook Chae, Han-Oh Park, and Dong-Myung Kim PLoS One. 2012; 7(9): e45023. More
S. cerevisiae
VN-Fusion Library
in vivo quantification of protein-protein interactions in Saccharomyces cerevisiae using bimolecular fluorescence complementation assay Min-Kyung Sung, Won-Ki Huh J Microbiol Methods. 2010. 83(2), 194-201. More
S. cerevisiae
VN-Fusion Library
Bimolecular fluorescence complementation analysis system for in vivo detection of protein-protein interaction in Saccharomyces cerevisiae Min-Kyung Sung, Won-Ki Huh Yeast. 2007. 24(9), 767-775. More
S. cerevisiae
VN-Fusion Library
Construction, verification and experimental use of two epitope-tagged collections of budding yeast strains Russell Howson, Won-Ki Huh, Sina Ghaemmaghami, James V. Falvo, Kiowa Bower, Archana Belle, Noah Dephoure, Dennis D. Wykoff, Jonathan S. Weissman and Erin K. O’Shea Comp Funct Genom. 2005. 6, 2–16. More
S. cerevisiae
VN-Fusion Library
Global analysis of protein localization in budding yeast Won-Ki Huh, James V. Falvo, Luke C. Gerke, Adam S. Carroll, Russell W. Howson, Jonathan S. Weissman and Erin K. O’Shea Nature. 2003. 425, 686-691. More
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