Fast micro scale purification of 6xHis-tagged protein
Bioneer's Ni-NTA magnetic silica resins are silica beads, with an average diameter of 1.29 um and a range of 1~5 um diameter, have Ni-NTA (nickel-nitrilotriacetic acid) covalently bound to their surface.
These Ni-NTA magnetic silica beads exhibit outstanding affinity to His-tagged proteins and feature our SSMB (Spherical-Shaped Magnetic Bead) technology that provides increased surface area (due to the small size of the beads), without the issue of impurity carryover common with magnetic particles that have a rough-surface.
Ni-NTA magnetic agarose beads are supplied as a 10% (v/v) suspension with an average binding capacity of 500 ug protein per ml of suspension for 6xHis-tagged protein.
Features and Benefits
-
Flexible: The protocol may be carried out with a magnet or via centrifugation.
-
Excellent performance: Highly pure proteins are obtained through our exclusive SSMB (Spherical-Shape Magnetic Beads).
-
High purity and High yield: Average binding capacity of 500 μg protein per ml of suspension for 6xHis-tagged protein. Purity that is > 90%.
-
Reproducibility: The only hands-on step is the addition of protein extract, maximizing reproducibility.
-
Fast process: The entire purification is complete in about 30 minutes!
]]>
Manual
• AccuPrep® His-tagged Protein Purification Kit
MSDS
• AccuPrep® Ni-NTA binding-washing buffer
• AccuPrep® Ni-NTA Elution buffer
Brochure
• AccuPrep® His-tagged Protein Purification Kit
Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
• ISO 9001:2008 - certificate
• EN ISO 13485:2003 / AC:2009 - certificate
]]>