This Protein size marker contains sample buffer, and it can be loading directly after heating at 95°C for 5 minutes.
AccuLadder™ Protein Size Marker (Broad) consist of 8 kinds of proteins (6.5kDa ~ 116kDa).
This Protein size marker contains sample buffer, and it can be loading directly after heating at 95°C for 5 minutes. 5ul of marker is loaded per lane of SDS-PAGE minigel. In this case, this marker is loaded for 100 lanes.
Features and Benefits
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Concentration: 0.1mg/mL ~ 0.2mg/mL
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Recommended loading: 5 ul / lane ( Minigel ; 10x8(cm2), 0.75 or 1.0mm thick )
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Size Range (kDa): 6.5 ~ 116 (kDa)
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Components
 |
|
Protein |
MW (kDa) |
Source |
|
β-Galactosidase |
116 |
E. coli |
|
Phosphorylase b |
97.4 |
Rabbit muscle |
|
Albumin |
66 |
Bovine serum |
|
Ovalbumin |
45 |
Chicken egg white |
|
Carbonic anhydrase |
29 |
Bovine erythrocytes |
|
Trypsin inhibitor |
20.1 |
Soybean |
|
Lysozyme |
14.4 |
Chicken egg white |
|
Aprotinin |
6.5 |
Bovine lung |
|
Storage conditions
-20°C
Notes
The Protein marker can be applied onto a SDS-PAGE gel.
Do heat before loading (95°C, 5min).
Repeated freezing and thawing should be avoided.
Experimental Data
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Manual
• AccuLadder™ Protein Size Marker (Broad)
MSDS
• AccuLadder™ Protein Size Marker (Broad)
Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
• ISO 9001:2008 - certificate
• EN ISO 13485:2003 / AC:2009 - certificate
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