• Gene Cloning


Gene cloning and subcloning of genes into any cloning vector you want-100% sequence guaranteed.

Let us perform the time-consuming task of gene cloning, subcloning and sequence verification. Bioneer brings years of experience in molecular biology together with state-of-the-art QC to guarantee the quality of custom gene cloning service.


Features and Benefits

  • 100% Sequence Guarantee: Individual custom clones are 100% confirmed by sequencing
  • Value Pricing: The best value for your research dollar
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Applications


Antibody Construction
Antibodies targeted toward specific diseases or targets can be constructed for maximum expression in the host organism. Also, an antibody library can be constructed to screen for the most efficient antibody variant.
 

Organism Production Optimization
Optimize expression of genes related to resource production to maximize industrial biological production efficiency.
 

Gene Construction
Get difficult-to-obtain DNA sequences without a template and upgrade the quality of your research by constructing hypothetical genes.
 

Protein Modification
Codon optimization can increase protein expression efficiency, and a mutant library derived from this process can yield proteins with increased function. Optimizations include secondary structure removal, and repeat reduction as well as organism optimizations.
 

Schematics of Gene Synthesis Process


 

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MSDS

Gene Synthesis Service


Brochure

Gene Synthesis Service


Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

ISO 9001:2008 - certificate

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Download  Gene Synthesis Order Form

Gene Cloning Service
Accepted Materials Plasmid, PCR product, Cultured E. coli cell
Price $200.00/cloning (~ 1kb insert / ~ 6kb vector)
Additional Costs With cultured cells $ 50.00
Insert $ 50.00 / 500bp (>1kb insert)
Vector 6 ~ 8kb: $ 50.00
8 ~ 10kb: $ 150.00
10kb ~ : inquire
Service Period 1 ~ 6kb(vector+insert) ~ 10 working days
6 ~ 8kb(vector+insert) 10 ~ 15 working days
8 ~ 10kb(vector+insert) 15 ~ 30 working days
10kb ~ (vector+insert) Inquire
Additional Service Produce high yield of plasmid DNA ($100.00/100 ug)
Proceed with Gene synthesis ($100.00 discount)

* Additional charges will apply for gene segments containing complexities such as high or low GC content, repeat sequences or homopolymeric runs. If any of your sequence is found to contain the above listed complexities, you will be contacted by our Gene Synthesis Specialist.
* When choosing the subcloning service with a commercial vector, a vector purchase cost will be billed separately.
* Synthesized genes will be sequenced. Only 100% sequence identity products will be shipped.
* 50% charge of service bill for cancellation in 5days.
80% charge of service bill for cancellation after 5days.
Free of charge for cancellation after guarantee duration(Guarantee duration: gene synthesis period except delivery)


Quotations and Ordering

1. Download the ordering form(s) from the link on top.
2. Fill out the form and email the form to: contact@bioneer.com.au
3. We will review the order and send you service information (final quote and service period) via email.
4. If you decide to go ahead with the service, please confirm the service by an email.
5. The service will be initiated when we send out the service start alert email.
(For the Cloning and Point mutation services, the service begins when we receive the raw material from you.)
* Please fill in the personal information columns (contact information).
We need it to fine-tune the service and expedite the order.

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