RNA Extraction – AccuZol™ Total RNA Extraction Solution
AccuZol™ is a ready-to-use reagent for the isolation of total RNA from various sample materials.
A monophasic solution of phenol and guanidine-salt inhibits RNase activity, and so the reagent maintains integrity of the RNA during sample homogenization or lysis.
AccuZol™ allows you to extract high-yield total RNA with small quantities of starting materials.
Features and Benefits
Simplicity
AccuZol™ minimize the steps handle hazardous reagents so that RNase/DNase contamination keeps to a minimum.
Experiment Time
The procedure for total RNA isolation can be completed in 1 hour.
Sample Source
Tissues and cultured cells originated from human, animals, plant, and bacteria, or blood samples.
Easy Phase Separation
Green colored reagent is useful to distinguish between organic phase and aqueous phase.
Application
cDNA synthesis, RT-PCR, Real-time RT-PCR, Northern/dot blot analysis, RNase protection assays, Molecular cloning
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Specifications
|
Samples |
Starting volume |
Elution volume |
Average yield (ug) |
Animal Tissue |
Liver
Kidney
Spleen
Lung
Brain |
10 mg |
50ul |
50 - 70
25 - 40
40 - 60
15 - 25
8 - 12 |
Cultured Cell |
HeLa
A549
PC3 |
1 x 106 cells |
50ul |
15 - 30
15 - 25
15 - 25 |
Bacterial Cell |
E.coli |
1 x 107 cells |
50ul |
10 - 15 |
Plant Tissue |
Bean leaf |
100 mg |
50ul |
30 - 50 |
Blood |
Human whole blood |
250 ul |
50ul |
2 - 3 |
Applications
cDNA synthesis, Northern blotting, RT-PCR, real time RT-PCR
Procedure
Experimental Data
Fig. 1 Electrophoresis Pattern of total RNA extracted from animal tissues by AccuZol™
Total RNA was isolated from 10-20mg of rat tissues and 2 ug of total RNA was loaded per lane on a 1% denaturing agarose gel.
Fig. 2 Electrophoresis Pattern of total RNA extracted from human cell lines by AccuZol™
Total RNA was isolated from 1x106 cultured cells and 1 ug of total RNA was loaded per lane on a 1% denaturing agarose gel.
Fig. 3 Electrophoresis Pattern of total RNA extracted from plant by AccuZol™
Total RNA was isolated from 100mg of bean leaves and 1 ug of total RNA was loaded per lane on a 1% denaturing agarose gel.
Fig. 4 RT & PCR analysis of total RNA extracted from rat tissues by AccuZol™
This analysis was performed with RT/PCR Premix (Bioneer, K-2055) and rat GAPDH primer. The electrophoresis pattern shows clear bands PCR products
Fig. 5 RT & PCR analysis of total RNA extracted from human cell lines by AccuZol™
This analysis was performed with RT/PCR Premix (Bioneer, K-2055) and human GAPDH primer. The electrophoresis pattern shows clear bands PCR products.
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Manual
• AccuZol™ Total RNA Extraction Solution
Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
• ISO 9001:2008 - certificate
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