Specifications
Starting culture volume |
up to 1X109 cells |
Elution volume |
50 - 100 ul |
Expected Yield |
up to 10 ug |
Expected Purity (A260/280) |
> 1.8 |
Applications
PCR, Quantitative real time PCR, Gene cloning, Genetics, Classification of bacteria
Procedure
Experimental Data
Fig. 1 Agarose gel electrophoresis results of genomic DNA extracted from E.coli cell(1X109 cells)
Results of gel electrophoresis of genomic DNA extracted from E.coli cell(1X109 cells). Yields were on average 8-12ug and purities (A260/A280) were at least (average) 1.8. Also, to observe cross-contamination that may occur during the extraction process, the E.coli cell(1X109 cells) were placed in a checkerboard pattern, and the rest of the wells were filled with D.W. No cross-contamination could be detected. M; Size marker, S; Extraction with E.coli cell(1X109 cells) sample, B; Extraction with D.W only.
Fig. 2 Real-time PCR results(MTB & NTM) of genomic DNA extracted from sputum samples
Real-time PCR results using an AccuPower® MTB & NTM EX Real-Time PCR Kit(MTN-1111, Bioneer) and run on Exicycler™ 96 Real-Time Quantitative Thermal Block (A-2060, Bioneer) from genomic DNA extracted from 10ml of sputum samples.
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Application Note
• ExiProgen™ Bacteria Genomic DNA Kit_E.coli cell
• ExiProgen™ Bacteria Genomic DNA Kit_M.tuberculosis
Manual
• ExiProgen™ Bacteria Genomic DNA Kit
Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
• ISO 9001:2008 - certificate
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