Specifications
5' to 3' exonuclease: No
3' to 5' exonuclease: No
3’ – A Overhang: No
Fragment size: 6 kb
Application
cDNA synthesis followed by PCR
Figure 1. Specific amplification of 5' - UTR region of HCV with AccuPower® RT-PCR PreMix.
Human DNA was amplified to generate 0.1-1.5 kb PCR fragments using 1 U of polymerase. The comparison was performed under the same experimental conditions.
Lane 1: DNA Ladder (BM corp.)
Lane 2: Negative control
Lane 3, 4: HCV positive serum
Lane 5: HCV negative serum
Figure 2. Sensitivity comparison between AccuPower® RT/PCR PreMix and Supplier I RT-PCR Kit
Each 109 copy – 103 copy of PSTVd used for RT/PCR and the same amount of RT/PCR products used for electrophoresis
Lane1: 109 copy Lane2: 108 copy
Lane3: 107 copy Lane4: 106 copy
Lane5: 105 copy Lane6: 104 copy
Lane7: 103 copy Lane8: NTC
Figure 3. Comparison of reproducibility test for AccuPower RT/PCR PreMix batch 1,2,3 and batch 4 products using serial diluted Human Total RNA.
Each 109 copy – 103 copy of PSTVd used for RT/PCR and the same amount of RT/PCR products used for electrophoresis
Lane 1: 10 ng Human total RNA from HeLa cell
Lane 2: 1 ng Human total RNA from HeLa cell
Lane 3: 100 pg Human total RNA from HeLa cell
Lane 4: 10 pg Human total RNA from HeLa cell
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Manual
• AccuPower® RT-PCR PreMix
MSDS
• AccuPower® PCR Master Mixes
Brochure
• AccuPower® PCR Master Mixes - 2010 Brochure
Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
• ISO 9001:2008 - certificate
• EN ISO 13485:2003 / AC:2009 - certificate
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