AccuPower® RT-PCR PreMix


cDNA synthesis followed by PCR in one tube



AccuPower® RT-PCR PreMix contains all the components necessary for sequential cDNA synthesis and amplification in one tube (one-step RT-PCR). This RT-PCR PreMix consists of both M-MLV Reverse Transcriptase, RNA dependent DNA polymerase, and a thermostable DNA polymerase in a lyophilized mix of dNTPs, reaction buffer, RNase inhibitor, tracking dye, and stabilizer. The kit can be used for double stranded cDNA synthesis from low copy RNA or mRNA and subsequent RT-PCR .


Features and Benefits

  • Convenient lyophilized RT: Easy to use, simply add your purified RNA
  •  High yield of cDNA: For genes up to 6 kb
  •  Stable for 2 years at -20°C: Long life
  •  RNase, DNase and Proteinase-free: Ensures the integrity of your samples
     
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Specifications

5' to 3' exonuclease: No
3' to 5' exonuclease: No
3’ – A Overhang: No
Fragment size: 6 kb

 

Application

cDNA synthesis followed by PCR


Figure 1. Specific amplification of 5' - UTR region of HCV with AccuPower® RT-PCR PreMix.
Human DNA was amplified to generate 0.1-1.5 kb PCR fragments using 1 U of polymerase. The comparison was performed under the same experimental conditions.

Lane 1: DNA Ladder (BM corp.)
Lane 2: Negative control
Lane 3, 4: HCV positive serum
Lane 5: HCV negative serum
 


Figure 2. Sensitivity comparison between AccuPower® RT/PCR PreMix and Supplier I RT-PCR Kit
Each 109 copy – 103 copy of PSTVd used for RT/PCR and the same amount of RT/PCR products used for electrophoresis

Lane1: 109 copy Lane2: 108 copy
Lane3: 107 copy Lane4: 106 copy
Lane5: 105 copy Lane6: 104 copy
Lane7: 103 copy Lane8: NTC
 


Figure 3. Comparison of reproducibility test for AccuPower RT/PCR PreMix batch 1,2,3 and batch 4 products using serial diluted Human Total RNA.
Each 109 copy – 103 copy of PSTVd used for RT/PCR and the same amount of RT/PCR products used for electrophoresis

Lane 1: 10 ng Human total RNA from HeLa cell
Lane 2: 1 ng Human total RNA from HeLa cell
Lane 3: 100 pg Human total RNA from HeLa cell
Lane 4: 10 pg Human total RNA from HeLa cell



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Manual
AccuPower® RT-PCR PreMix


MSDS
AccuPower® PCR Master Mixes


Brochure
• AccuPower® PCR Master Mixes - 2010 Brochure


Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.

ISO 9001:2008 - certificate

EN ISO 13485:2003 / AC:2009 - certificate

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